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Figure 1 | BMC Plant Biology

Figure 1

From: A strong constitutive ethylene-response phenotype conferred on Arabidopsis plants containing null mutations in the ethylene receptors ETR1 and ERS1

Figure 1

Analysis of the ers1-3 and etr1-9 T-DNA insertion alleles. (A) Positions of T-DNA insertions. Arrows indicate site of primers used for RT-PCR analysis. (B) Northern blot analysis. Poly-A RNA from wild type (WT), etr1-9, and ers1-3 were probed for the expression of ETR1, ERS1, or the control β-tubulin. (C) RT-PCR analysis for expression from ETR1. Expression was analyzed in wild type (WT) and in etr1-9 backgrounds using primers specific for sequences 5' and 3' to the site of the T-DNA insertion. Genomic DNA (WTg) was included to confirm the difference in PCR product sizes from cDNA and genomic DNA templates. Ubiquitin (UBQ) was used as a control. (D) Immunoblot analysis for expression of ETR1. Membranes from wild type and mutant lines were probed with antibodies against different regions of ETR1, anti-ETR1(165–401) and anti-ETR1(401–738), as well as with the anti-(H+-ATPase) antibody as a loading control. Wild-type ETR1 migrates at a molecular mass of 77 kDa. The asterisk indicates a nonspecific protein of 65 kDa that cross reacts with the anti-ETR1(165–401) antibody but not with anti-ETR1(401–738) antibody. The predicted migration position of the hypothetical truncated receptor (58 kDa) is indicated.

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