Expression of eqFP611 and smGFP4 fusion proteins in N. benthamiana after leaf infiltration. Agrobacterium-mediated transformation of N. benthamiana wild-type leaves with a construct encoding both eqFP611 and smGFP4 with mitochondrial presequences (pIVD144-eqFP611-IVD145-smGFP4-pBI121) on a single plasmid. Images of epidermal cell layers taken through MitoTracker (A) and FITC (B) filter sets, respectively. (C) Image taken through FITC filter set with addition of white light. Some mitochondria are examplarily indicated by a white arrow. Scale bars: 10 μm. (D) Representation of the plasmid used for agroinfiltration. The IFP expression cassettes are identical with those in Figure 6, but have been inserted into pBI121. Kanr: kanamycin resistance cassette (NOS promoter, neomycin phosphotransferase II, NOS terminator), RB: right border, LB: left border. Vector backbone: pBI121.