Co-expression of peroxisomally targeted eqFP611 and mitochondrially targeted smGFP4. Co-transfection of N. tabacum wild-type protoplasts with two separate plasmids encoding eqFP611 with a peroxisomal targeting signal 2 (pKAT2-eqFP611) and smGFP4 with a mitochondrial presequence (pIVD145-smGFP4). Images of a cell transfected with both constructs through MitoTracker (A) and FITC (B) filter sets, respectively. Scale bars: 10 μm. (C) Plasmids pKAT2-eqFP611 and pIVD145-eqFP611 used for transfection. Black arrow, CaMV 35S: cauliflower mosaic virus 35S promoter; grey box, KAT2: N-terminal 297 nucleotides of the KAT2 coding sequence; grey box, IVD(145): N-terminal 145 nucleotides of the IVD coding sequence; red box, eqFP611: eqFP611 coding sequence; green box, smGFP4: smGFP4 coding sequence; black box, NOS T: nopaline synthase terminator. H: HindIII, P: PstI, Xb: XbaI, Sm: SmaI, Sa: SacI, E: EcoRI, B: BamHI restriction sites. Vector backbone: pUC19.