Figure 1

Gene Ontology annotation of Centaurea Unigenes. A normalized cDNA library was created from whole plants representing seven invasive populations of Centaurea maculosa. Five thousand ESTs were sequenced from the 5' end (Agencourt biosciences), and assembled into 4,423 contigs, or 'Centaurea unigenes.' Unigenes were translated in all frames and the resulting amino acid sequences were used as BLAST queries. Top BLAST hits provided annotation and functional categorization (gene ontology assignment) for each Centaurea unigene. Not all unigenes were able to be annotated by GO programs. Computational analysis was done using the PLAN database (Noble Foundation).