Figure 5

Identification of transcripts homologous to Lycopersicon EPRVs. A. Survey over a selection of homologous ESTs of the genus Solanum subsection Lycopersicon and their position (grey boxes) in relation to the LycEPRV structure. Details about the ESTs (according to the numbers) are given in Table 3. Arrows mark the position of primers used for RT-PCR. B. PolyA⁺-enriched RNA of S.lycopersicum, S.habrochaites and an interspecific hybrid was used for RT-PCR with primer pairs of the CP/MP and TAV ORFs and the IGR as indicated in (A). The first strand DNA template was prepared from polyA⁺ enriched RNA from leaves of S.lycopersicum, S.habrochaites and an interspecific hybrid (lane 1–3 each). To detect possible genomic DNA contaminations an actin sequence spanning an intron was amplified in parallel. Water controls are indicated by a dash (lane 4 each). C, D. Unrooted dendrograms showing the genetic distance between genomic and cDNA sequences of the TAV region (C) and the IGR (D) of S.lycopersicum (red boxes), S.habrochaites (green boxes) and an interspecific hybrid (white boxes). cDNA sequences are indicated by a square, circles mark genomic sequences. The horizontal bar represents percent divergence (/100).