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Table 2 Summary of cDNA libraries.

From: Analysis of gene expression in cotton fiber initials

Description

no. EST

unique contigs

singletons

cultivar

Accession numbers

fiber 5 dpa (FFT)

274

16

59

DES119

DW223534–DW223807

lower stem (between the root and first leaf node 7 weeks after planting)

1029

12

163

DES119

DW223808–DW224836

lower stem (between the root and first leaf node 3 weeks after planting)

3203

22

287

DES119

DW224837–DW228039

fiber initials 1 dpa (from membrane bound polyribosomes)

1641

24

71

DES119

DW228040–DW229680

0 dpa ovules from the fiberless mutant (SL1-7-1)

1727

37

156

SL1-7-1

DW229681–DW231407

0 dpa ovules from DES119

1749

0

148

DES119

DW231414–DW233162

root 3 week after planting (from free polyribosomes)

2081

14

191

DES119

DW233163–DW235243

root 3 week after planting (from membrance bound polyribosomes)

669

29

82

DES119

DW235244–DW235912

Roots 10–12 weeks after planting

2818

27

222

DES119

DW235913–DW238730

stems 7 weeks after planting (membrane bound polyribosomes)

519

5

4

DES119

DW238731–DW239249

stems 3 week after planting

3087

0

282

DES119

DW239250–DW242336

stems 3 week after planting (from membrane bound polyribosomes)

690

0

88

DES119

DW242337–DW243026

stems 3 weeks after planting (unsuccesful normalization)

1036

0

63

DES119

DW243027–DW244062

Young fiber (1–5 dpa)

1583

0

10

DES119

DW244063–DW245645

random primed normalized

19600

704

3325

DES119

DW476068–DW495667

oligo dT primed normalized

25015

675

3450

DES119

DW495668–DW520682

  1. Description of cDNA libraries, the number of ESTs sequences in each library, unique contigs composed exclusively of ESTs from library, singletons unique to library, cotton cultivar used to make cDNA, GenBank accession numbers of ESTs sequences.