Figure 1

RNA isolated from frozen ovules. One sample was mixed with glass beads and vortexed and a picture was taken. A picture was taken of the other frozen ovules without addition of glass beads or vortexing (A). Polyribosomal RNA was isolated from vortexed 1 dpa, 3 dpa, 5 dpa or 7 dpa ovules and shoots. Free-polyribosomal (F) or membrane bound polyribosomal (Mb) RNA was isolated from vortexed 3 dpa ovules. The RNA was separated on a 1.2% gel and visualized by staining with ethidium-bromide.