Figure 3

Protein sequence alignment of SRF proteins. SRF1A' represents the Ler version of SRF1A. All other sequences correspond to Col. Individual protein domains are indicated above the sequences. Full conservation across the alignment is marked by black columns, partial conservation by gray columns. Red color highlights a non-conservative residue exchange. Blue color marks a conservative or semi-conservative amino acid exchange. 3a) Alignment of predicted SRF amino acid sequences up to and including the transmembrane domain. Individual protein domains are indicated above the sequences. The predicted signal peptide sequences and the transmembrane domains are underlined with thick black lines. The proline-rich regions are underlined with thin black lines. Predicted PEST sequences are kept in italic. 3b) Alignment of predicted SRF amino acid sequences from juxtra-membrane domain up to and including the C-terminus. Asteriks highlight important kinase residues as revealed by standard kinase alignments [38]. SRF1: the cross in the juxtamembrane region marks the point of deviation of the SRF1A/B sequences. The proline-rich region of SRF3 is underlined with a thick black line. The 12 kinase subdomains are indicated. The in vivo phosphorylation sites of SRF3 (S. Peck, pers. communication), SRF7, and SRF8 are highlighted in italics and are underlined.