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Table 1 List of Physcomitrella miRNAs identified by cloning.

From: Evidence for the rapid expansion of microRNA-mediated regulation in early land plant evolution

Name

Sequence 5'→3'

Length (nt)

Homolog to known miRNA

Precursor genomic

Precursor EST

Expression verified

1–22

AUUGGGACUUGUGCUGGGAC

20

 

1 (gnl|ti|872730449)

n.f.

no

1–39

CGUUUCACGUCGGGUUCACC

20

 

1 (gnl|ti|713836555)

n.f.

no

1–50

UGGCUGAGUCGAAGGUUGUGC

21

 

1 (gnl|ti|859644225)

n.f.

yes (P/G)

1–63

UUGCUGUGCACUACUUAGUA

20

 

2 (gnl|ti|1012878547, gnl|ti|835906822)

1 (PR_1-63/3-14)

yes (P/G)

2-1

GUAGCUUAGCGAGGUGUUGGUA

22

 

1 (gnl|ti|890552627)

1 (PR_2-1)

yes (G)

2–28

CGCUGUCCAUUCUGAGCAUUG

21

 

1 (gnl|ti|1010151671)

n.f.

yes (P/G)

2–31a

UGACAACGAGAGAGAGCACGC

21

miR535 (ppt, osa)

3 (gnl|ti|1003237208, gnl|ti|756805268, gnl|ti|872833603)

n.f.

n.e.

2–42

GUCAAUUUGGCCGAGUGGUUAAGGC

25

 

1 (gnl|ti|1000320159)

n.f.

n.e.

2–51

GAGCUUUCUUCGGUCCAAUA

20

 

1 (gnl|ti|859644225)

n.f.

yes (P)

2–86a

CCUUAGAGUCGUAGGCCUCUG

21

miR1218 (ppt)

1 (gnl|ti|774610216)

1 (PR_2-86)

n.e.

2–88a

UGACAGAAGAGAGUGAGCAC

20

miR156 (ath, osa, zma, sbi, sof, gma, ptc, ppt)

2 (gnl|ti|850661024, gnl|ti|784299453)

n.f.

n.e.

3–5

UGAUCAAGUGGAAACUCAGCAAA

23

 

1 (gnl|ti|863031657)

n.f.

no

3–14

GCUAGGCAGUGCACAGCGAUA

21

 

1 (gnl|ti|1012878547)

1 (PR_1-63/3-14)

yes (P/G)

3–60a

UUCGUGCCAAGCUGUGUGCAAC

22

miR536 (ppt)

2 (gnl|ti|890625113, gnl|ti|869792930)

1 (PR_3-60)

n.e.

3–62

AACUGAGAUACAUCGCAAUCG

21

 

1 (gnl|ti|1029072876)

n.f.

no

3–91

GCUGUGUUCUUGUACCUGGG

20

 

1 (gnl|ti|831706876)

n.f.

no

5–21

UCUUGUCAAUGUUUAGGGGC

20

 

2 (gnl|ti|891393071, gnl|ti|836345675)

n.f.

yes (P/G)

5–33a

UUGAGGUGUUUCUACAGGCU

20

miR537 (ppt)

1 (gnl|ti|903313912)

n.f.

n.e.

4–12

GGUAAAGUGGCGGCUAGGUUA

21

 

1 (gnl|ti|890397681)

n.f.

no

4–34a

CGUGGGACAGCAUAGAAUGCG

21

miR1212 (ppt, pj)

1 (gnl|ti|713871562)

n.f.

n.e.

4–66

ACGAAGGUCUGCAUCAUAGCCAA

23

 

2 (gnl|ti|1000325696, gnl|ti|816375179)

1 (PR_4-66)

yes (P)

4–67b

AUCGUGCCAAGCUUUGUGCUUU

22

miR536 (ppt)

1 (gnl|ti|713832028)

n.f.

n.e.

4–72b

UUGAGCCGCGCCAAUAUCACA

21

miR171 (ath, zma, osa, ptc)

2 (gnl|ti|1023219413, gnl|ti|993696673)

n.f.

yes (P)

3–36

GCUACUUCGGCGGGACAAGAGA

22

 

1 (gnl|ti|1020603193)

n.f.

n.e.

2–70

GUUGGAAGCCUUCGUGGGA

19

 

n.f.

1 (PR_2-70)

no

2–15b

UGACAACGAGAGAGAGUACGCU

22

miR535 (ppt, osa)

n.f.

n.f.

n.e.

3–40b

UGACAGAAGAGAGUGAGCACAU

22

miR156 (ath, gma, mtr, osa ptc, sbi, sof, zma, ppt)

n.f.

n.f.

n.e.

3–44c

UCGGAAGCCUUUGUGGGAGAGGAA

24

miR477 (ptc)

n.f.

n.f.

yes (P/G)

3–54b

CUUGGACUGAAGGGAGCUUUUUUU

24

miR319 (ath, gma, ppt, ptc, sbi, sof, zma)

n.f.

n.f.

yes (P/G)

3–79c

UCAUCCAGGGAGCCAGACAGA

21

miR160 (ath, gma, mtr, ptc, osa, sbi, zma)

n.f.

n.f.

no

  1. a Identical to previously identified miRNA. b Homologous to known miRNA family, but not identical to individual members of this family. c The reverse and complementary sequence of the miRNA shows similarity to known miRNAs. ath: Arabidopsis thaliana, gma: Glycine max, mtr: Medicago truncatula, osa: Oryza sativa, ptc: Populus trichocarpa, ppt: Physcomitrella patens, pj: Polytrichum juniperinum, sbi: Sorghum bicolor, sof: Saccharum officinarum, zma: Zea mays. Underlined accession numbers of genomic sequences indicate an identity > 95% to the EST sequence. n.e.: not examined, n.f.: not found. P: expressed in protonema tissue, G: expressed in gametophore tissue.