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Table 4 Primers used

From: Silencing of beta-carotene hydroxylase increases total carotenoid and beta-carotene levels in potato tubers

Primer name

Sequence

Use

AS-chy Up1

TAGAGCTCGGGATTACTTC

AS-chy cloning

AS-chy Dw2

ATGGATCCTCCTTTTCCAA

AS-chy cloning

AS-h Up

GTTAAGGGAACTTCTCCAC

PCR screening

Nos-test 2

CGCGTATTAAATGTATAATTG

PCR screening

AS-h RT Up

ACCCTCCATTTGCCACGAA

Real-time assay

AS-h RT Dw

TTATATGATAATCATCGCAAGACCG

Real-time assay

Chy1 Up

CTTGGCCCAAAACCCACTT

Real-time assay

Chy1 Dw

CCTCAAATTGAGGTTTCAGCTTCT

Real-time assay

Chy2 Up

TTTTGCTGTCTCGAAGAAAGCC

Real-time assay

Chy2 Dw

AGCCAACAGGCAGCTAAACTCT

Real-time assay

Lut5 Up

GTCTCAAGCAAGCAACTTCGTG

Real-time assay

Lut5 Dw

GATAAAAGGTCCATGTGAGCACTG

Real-time assay

Nxs Up

CTTGGAGGAGACTTCTTTGGTGA

Real-time assay

Nxs Dw

CGGAAGTGGTCCTCCCATAG

Real-time assay

  1. Sequences of the primers used for cloning of the gene fragment, for PCR screening of the putative transgenic plants, and for Real Time RT-PCR quantitation of transcript levels. For further details, see Methods.