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Table 2 HPLC analysis of tuber carotenoids (ng/g dry weight)

From: Silencing of beta-carotene hydroxylase increases total carotenoid and beta-carotene levels in potato tubers

  Phytofluene Lutein β-carotene Zeaxanthin Antheraxanthin Violaxanthin Neoxanthin Other Xanth. Esters Total
Wild-type 61.69 ± 5.60 426.00 ± 124.44 2.25 ± 1.17 320.87 ± 111.63 1415.67 ± 335.02 1598.73 ± 468.72 468.76 ± 161.43 76.91 ± 22.60 517.06 ± 178.15 4887.95 ± 1421.16
Gus 2 54.30 ± 5.28 501.81 ± 126.33 3.11 ± 2.15 311.42 ± 98.85 1317.06 ± 149.48 1282.35 ± 403.34 488.24 ± 121.55 91.14 ± 21,55 615.01 ± 98.73 4664.44 ± 1022.00
Fold Variation 0.88 1.18 1.38 0.97 0.93 0.80 1.04 1.18 1.19 0.95
As-h1 162.57 ± 2.88 1590.36 ± 257.09 85.30 ± 2.33 168.37 ± 308.48 2198.74 ± 983.80 3452.99 ± 749.84 1509.40 ± 269.05 177.44 ± 54.47 4918.76 ± 920.25 14263.95 ± 2992.78
Fold Variation 2.63*** 3.73 37.91*** 0.52* 1.55 2.16* 3.22*** 2.31* 9.51*** 2.92***
As-h2 55.64 ± 0.58 2637.17 ± 570.03 34.08 ± 11.71 147.90 ± 39.91 945.83 ± 156.85 802.98 ± 87.20 832.59 ± 240.51 367.85 ± 19.18 4587.20 ± 1069.79 10411.26 ± 2195.20
Fold Variation 0.90 6.19*** 15.15** 0.46* 0.67 0.50 1.78 4.78*** 8.87*** 2.13**
As-h3 74.70 ± 8.85 2982.04 ± 881.18 56.34 ± 16.90 37.09 ± 5.84 1034.68 ± 402.16 11422.26 ± 2470.47 754.32 ± 228.82 329.06 ± 87.63 5068.07 ± 1181.94 21758.57 ± 5274.94
Fold Variation 1.21* 7.00*** 25.04*** 0.12*** 0.73 7.14*** 1.61 4.28*** 9.80*** 4.45***
As-h4 67.64 ± 24.22 1808.90 ± 239.24 32.65 ± 6.50 153.71 ± 39.99 1102.94 ± 287.98 3377.31 ± 371.15 764.34 ± 171.39 255.47 ± 17.75 1789.33 ± 51.88 9352.31 ± 1186.71
Fold Variation 1.10 4.25*** 14.51** 0.48** 0.78 2.11 1.63 3.32*** 3.46 1.91**
As-h5 63.30 ± 5.84 764.54 ± 244.69 2.71 ± 7.75 218.53 ± 140.68 1382.88 ± 203.52 2018.71 ± 282.39 456.06 ± 55.76 126.36 ± 3.23 1293.42 ± 564.92 6326.51 ± 1502.94
Fold Variation 1.03 1.79 1.20 0.68 0.98 1.26 0.97 1.64 2.50 1.29
As-h6 73.12 ± 19.18 575.11 ± 71.52 4.31 ± 0.43 331.03 ± 28.14 1551.15 ± 108.39 1780.21 ± 509.03 617.62 ± 103.11 120.02 ± 35.45 924.89 ± 132.96 5977.47 ± 989.06
Fold Variation 1.18 1.35 1.92 1.03 1.10 1.11 1.32 1.56 1.79 1.22
  1. Carotenoids were measured via diode array HPLC (see Methods) on a minimum of 4 different tubers from 2 different plants. Fold variation with respect to the wild-type is reported for each carotenoid compound and for each line. Asterisks indicate significance of the fold variation in an ANOVA test (*: p ≤ 0.05;**: p ≤ 0.01; ***: p ≤ 0.001). For details, see Methods.