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Table 2 HPLC analysis of tuber carotenoids (ng/g dry weight)

From: Silencing of beta-carotene hydroxylase increases total carotenoid and beta-carotene levels in potato tubers

 

Phytofluene

Lutein

β-carotene

Zeaxanthin

Antheraxanthin

Violaxanthin

Neoxanthin

Other Xanth.

Esters

Total

Wild-type

61.69 ± 5.60

426.00 ± 124.44

2.25 ± 1.17

320.87 ± 111.63

1415.67 ± 335.02

1598.73 ± 468.72

468.76 ± 161.43

76.91 ± 22.60

517.06 ± 178.15

4887.95 ± 1421.16

Gus 2

54.30 ± 5.28

501.81 ± 126.33

3.11 ± 2.15

311.42 ± 98.85

1317.06 ± 149.48

1282.35 ± 403.34

488.24 ± 121.55

91.14 ± 21,55

615.01 ± 98.73

4664.44 ± 1022.00

Fold Variation

0.88

1.18

1.38

0.97

0.93

0.80

1.04

1.18

1.19

0.95

As-h1

162.57 ± 2.88

1590.36 ± 257.09

85.30 ± 2.33

168.37 ± 308.48

2198.74 ± 983.80

3452.99 ± 749.84

1509.40 ± 269.05

177.44 ± 54.47

4918.76 ± 920.25

14263.95 ± 2992.78

Fold Variation

2.63***

3.73

37.91***

0.52*

1.55

2.16*

3.22***

2.31*

9.51***

2.92***

As-h2

55.64 ± 0.58

2637.17 ± 570.03

34.08 ± 11.71

147.90 ± 39.91

945.83 ± 156.85

802.98 ± 87.20

832.59 ± 240.51

367.85 ± 19.18

4587.20 ± 1069.79

10411.26 ± 2195.20

Fold Variation

0.90

6.19***

15.15**

0.46*

0.67

0.50

1.78

4.78***

8.87***

2.13**

As-h3

74.70 ± 8.85

2982.04 ± 881.18

56.34 ± 16.90

37.09 ± 5.84

1034.68 ± 402.16

11422.26 ± 2470.47

754.32 ± 228.82

329.06 ± 87.63

5068.07 ± 1181.94

21758.57 ± 5274.94

Fold Variation

1.21*

7.00***

25.04***

0.12***

0.73

7.14***

1.61

4.28***

9.80***

4.45***

As-h4

67.64 ± 24.22

1808.90 ± 239.24

32.65 ± 6.50

153.71 ± 39.99

1102.94 ± 287.98

3377.31 ± 371.15

764.34 ± 171.39

255.47 ± 17.75

1789.33 ± 51.88

9352.31 ± 1186.71

Fold Variation

1.10

4.25***

14.51**

0.48**

0.78

2.11

1.63

3.32***

3.46

1.91**

As-h5

63.30 ± 5.84

764.54 ± 244.69

2.71 ± 7.75

218.53 ± 140.68

1382.88 ± 203.52

2018.71 ± 282.39

456.06 ± 55.76

126.36 ± 3.23

1293.42 ± 564.92

6326.51 ± 1502.94

Fold Variation

1.03

1.79

1.20

0.68

0.98

1.26

0.97

1.64

2.50

1.29

As-h6

73.12 ± 19.18

575.11 ± 71.52

4.31 ± 0.43

331.03 ± 28.14

1551.15 ± 108.39

1780.21 ± 509.03

617.62 ± 103.11

120.02 ± 35.45

924.89 ± 132.96

5977.47 ± 989.06

Fold Variation

1.18

1.35

1.92

1.03

1.10

1.11

1.32

1.56

1.79

1.22

  1. Carotenoids were measured via diode array HPLC (see Methods) on a minimum of 4 different tubers from 2 different plants. Fold variation with respect to the wild-type is reported for each carotenoid compound and for each line. Asterisks indicate significance of the fold variation in an ANOVA test (*: p ≤ 0.05;**: p ≤ 0.01; ***: p ≤ 0.001). For details, see Methods.
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BMC Plant Biology

ISSN: 1471-2229

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