Quantification of the tissue-culture induced variation in barley (Hordeum vulgare L.)

BMC Plant Biology

Table 5 Adapters and metAFLP oligomer sequences.

Adapters/Primers	Sequence 5'→3'
Acc65I adapter 1	CTC GTA GCA TGC GTA CA
Acc65I adapter 2	GTA CTG TAC GCA TGC TAC
KpnI adapter 1	CTC GTA GCA TGC GTA CAG TAC
KpnI adapter 2	ACT CGT ACG ACT GT
MseI adapter 1	TAC TCA GGA CTC ATC
MseI adapter 2	GAG TCC TGA GTA GCA G
Acc65I/KpnI pre-selective primer	GCA TGC GTA CAG TAC C
MseI pre-selective primer	GAT GAG TCC TGA GTA AC
CpG-GAC	CAT GCG TAC AGT ACC GAC
CpG-GCA	CAT GCG TAC AGT ACC GCA
CpG-GGC	CAT GCG TAC AGT ACC GGC
CpXpG-AGG	CAT GCG TAC AGT ACC AGG
CpXpG-AGA	CAT GCG TAC AGT ACC AGA
CpXpG-AGC	CAT GCG TAC AGT ACC AGC
CpXpG-TGC	CAT GCG TAC AGT ACC TGC
MCAA	GAT GAG TCC TGA GTA AC
MCAG	GAT GAG TCC TGA GTA AC
MCAC	GAT GAG TCC TGA GTA AC
MCGC	GAT GAG TCC TGA GTA AC
MCCA	GAT GAG TCC TGA GTA AC
MCCG	GAT GAG TCC TGA GTA AC

ISSN: 1471-2229