Figure 2From: Identification of amino acid residues involved in substrate specificity of plant acyl-ACP thioesterases using a bioinformatics-guided approachAmino acid sequence alignment of Arabidopsis thaliana FatA3 (NP_189147) with FatB3-2 (CAA85388). The first residue of the mature enzyme is marked with an arrow. The two hot-dog domains of the 3D structural model [11] are underlined. Completely conserved residues are underlined. Amino acid positions determined to be SDPs by previous authors are marked with filled circles. Asterisks denote the CPDL-identified putative specificity determining positions. + marks residues comprising the catalytic triad of C, H, N. X's mark positions where mutations inactivated the enzyme.Back to article page