Distinct short-range ovule signals attract or repel Arabidopsis thaliana pollen tubes in vitro

BMC Plant Biology

Table 1 In vitro ovule targeting efficiency

Female tissues				Fraction ovules targeted^a	%	n^b
Stigma	Style	Ovule	Funiculus
–	–	+	+	4/162	3*	6
+	+	+	+	77/145	54	18
+	–	+	+	30/156	19*	25
+	+	+	–	61/175	35*	21
+	+	+ (fertilized)^c	+	0/122	0*	20
+	+	+ (100°C)^d	+	0/52	0*	8
+	+	+ (25°C)^d	+	23/57	40	8

^aAlthough the same number of ovules were placed in each assay, only those with a pollen tube tip ≤ 100 μm from the micropyle were considered potential targets; ^bnumber of independent in vitro assays; ^cexpanded ovules, indicating embryo growth, were collected 1 day after pollination; ^dovules immersed in 100°C or 25°C water for 5 min; tissues present (+) or absent (–) after removal by microdissection (rows 1 and 4); assays in which pollen tubes germinated and grew only on the stigma (row 3); *, significantly different (χ², P < 0.001) from assays performed with a stigma, style and intact funiculus (row 2).

ISSN: 1471-2229