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Figure 5 | BMC Plant Biology

Figure 5

From: Gene amplification of the Hps locus in Glycine max

Figure 5

Segregation of Hps polymorphisms and seed lustre in a soybean cross. An analysis of a genetic cross between two different soybean lines polymorphic for Hps and seed lustre phenotype. Soybean (Glycine max) genomic DNA (30 μg each lane) was digested with Bgl II and separated by agarose gel electrophoresis. The DNA was transferred to Nylon blotting membrane and probed with 32P labelled Hps cDNA (GenBank accession number AF100159). Shown above each lane is: Lustre, the seed lustre for each parent or F3 family (F4 seed); Genotype, the genotype for molecular marker A963_CAPS [2] where A is homozygous for the Mukden allele, B is homozygous for the OX281 allele, and H is heterozygous. The parental names and F3 family numbers are also shown.

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