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Table 3 Substrate specificity of the purified rice Os4bglu12

From: Analysis of rice glycosyl hydrolase family 1 and expression of Os4bglu12 β-glucosidase

Substrate

Relative activitya(%)

Laminaribioseb

238

Laminaritriose

0

Laminaritetraose

0

Laminaripentaose

0

Cellobiose

0

Cellotrioseb

231

Cellotetraoseb

301

Cellopentaoseb

279

Cellohexaoseb

295

Gentiobiose

0

Laminarin

0

Barley 1,3, 1,4-β-glucans

0

pNP-β-D-glucoside

100

pNP-β-D-fucoside

118

pNP-β-D-galactoside

45

pNP-β-D-xyloside

45

pNP-α-L-arabionoside

26

pNP-β-D-mannoside

0

pNP-β-D-cellobioside

0

pNP-α-D-glucoside

0

pNP-β-L-fucoside

0

  1. aPercent activity relative to glucose or pNP released from pNP-β-D-glucoside. The assay contained 1 mM substrate in 50 mM sodium acetate (pH 5.0) buffer at 37°C. bNote that the values for oligosaccharides are in terms of total glucose released.