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Table 3 Substrate specificity of the purified rice Os4bglu12

From: Analysis of rice glycosyl hydrolase family 1 and expression of Os4bglu12 β-glucosidase

Substrate Relative activitya(%)
Laminaribioseb 238
Laminaritriose 0
Laminaritetraose 0
Laminaripentaose 0
Cellobiose 0
Cellotrioseb 231
Cellotetraoseb 301
Cellopentaoseb 279
Cellohexaoseb 295
Gentiobiose 0
Laminarin 0
Barley 1,3, 1,4-β-glucans 0
pNP-β-D-glucoside 100
pNP-β-D-fucoside 118
pNP-β-D-galactoside 45
pNP-β-D-xyloside 45
pNP-α-L-arabionoside 26
pNP-β-D-mannoside 0
pNP-β-D-cellobioside 0
pNP-α-D-glucoside 0
pNP-β-L-fucoside 0
  1. aPercent activity relative to glucose or pNP released from pNP-β-D-glucoside. The assay contained 1 mM substrate in 50 mM sodium acetate (pH 5.0) buffer at 37°C. bNote that the values for oligosaccharides are in terms of total glucose released.