Figure 6
From: Analysis of rice glycosyl hydrolase family 1 and expression of Os4bglu12 β-glucosidase
Hydrolysis of oligosaccharide substrates by Os4bglu12 detected by TLC. The Os4bglu12 was incubated with 5 mM substrates for 30 min and the products were detected after TLC by the carbohydrate staining. Samples were incubated with (+) or without (-) enzyme. Lanes: 1, glucose (G) and cello-oligosaccharides of DP 2–4 (C2-C4) marker; 2 and 3, cellobiose; 4 and 5, cellotriose; 6 and 7, cellotetraose, 8 and 9, cellopentaose, 10 laminari-oligosaccharides of DP 2–4 (L2-L4) marker; 11 and 12, laminaribiose; 13–14 laminaritriose.