Figure 5
From: Analysis of rice glycosyl hydrolase family 1 and expression of Os4bglu12 β-glucosidase
SDS-PAGE profiles of Os4bglu12 recombinant protein expressed in OrigamiB (DE3)E. coli after incubation in the presence of 0.3 mM IPTG, at 20°C for 8 h. Lanes: 1, standard marker (Bio-RAD); 2, total protein in E. coli cells containing pET32a(+) without an insert; 3, total protein of E. coli cells containing pET32a(+)/DEST-Os4bglu12; 4, soluble fraction of E. coli cells containing pET32a(+)/DEST-Os4bglu12; 5, purified Os4bglu12 recombinant protein. The arrow points to the position of thioredoxin fusion protein monomer.