Figure 2From: Lutein is needed for efficient chlorophyll triplet quenching in the major LHCII antenna complex of higher plants and effective photoprotection in vivounder strong lightPhotobleaching behaviour of isolated Lhcb. (A) Monomeric Lhcb isolated from solubilized thylakoids of WT and lut2.1, and trimeric LHCII from WT were analyzed by following the Qy-transition absorbance decay during strong illumination. (B) Sucrose bands 2 and 3 from WT and lut2.1 were fractionated by flat bed IEF in order to purify LHCII subunits in their monomeric and trimeric form. Kinetics of Qy-transition absorbance decay were measured on isolated complexes as described in Experimental Procedures. Chlorophyll concentrations of Lhcb were set to 8 μg/ml. Samples were cooled to 10°C during measurements.Back to article page