Skip to main content

Table 2 PCR primers used

From: Identification of microspore-active promoters that allow targeted manipulation of gene expression at early stages of microgametogenesis in Arabidopsis

Primer

Primer sequence

cDNA construction

3'-RACE

5'-AAGCAGTGGTAACAACGCAGAGTAC(T)30VN-3'

RT-PCR reverse primer

NESTED

5'-AAGCAGTGGTAACAACGCAGAGT-3'

RT-PCR forward primers

At5g40040

5'-CTTATCGCTGTTGGACGAGAGAAGA-3'

At5g59040

5'-TCTGTCTCGCCGTCATTTTTGTTAT-3'

At5g46795

5'-GGCTTTGGAGACCAGACTTTTTCAG-3'

At4g26440

5'-TGGAGAGGTAGAAGAGTCCGAATCA-3'

At2g03170

5'-AGAAACACGTCGTTGACGAAGAAAG-3'

At3g14450

5'-GGCCAAGGAGTTTTTCCCTTCTTA-3'

At1g53650

5'-CTCGATCATTGAGCTCAGAAGCTGT-3'.

Construction of promoter::GUS reporters

MSP1-F

5'-AAAAAGCAGGCTTGTCAGTTAGCATGAAAAATTGTATGTTAG-3'

MSP1-R

5'-AGAAAGCTGGGTTTGTTGTGTATACTTGTGTGTGTGTATTTA-3'

MSP2-F

5'-AAAAAGCAGGCTATGTCCTACGATCAGAAGGAGGAG-3'

MSP2-R

5'-AGAAAGCTGGGTAACATGTGATATTATTTTTTTGGTTTATATAGTGG-3'

MSP3-F

5'-AAAAAGCAGGCTTTGTGATATAATAGGTATATATGGTAGAAC-3'

MSP3-R

5'-AGAAAGCTGGGTTGCAAACCCAAGTTTCAGCTTTAAC-3'

  1. Primers used for cDNA synthesis, RT-PCR analyses and construction of promoter::GUS reporters. Where appropriate, appended adapters complementary to AttB1 and AttB2 primers are underlined.
Back to article page

BMC Plant Biology

ISSN: 1471-2229

Contact us