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Table 2 PCR primers used

From: Identification of microspore-active promoters that allow targeted manipulation of gene expression at early stages of microgametogenesis in Arabidopsis

Primer Primer sequence
cDNA construction
3'-RACE 5'-AAGCAGTGGTAACAACGCAGAGTAC(T)30VN-3'
RT-PCR reverse primer
NESTED 5'-AAGCAGTGGTAACAACGCAGAGT-3'
RT-PCR forward primers
At5g40040 5'-CTTATCGCTGTTGGACGAGAGAAGA-3'
At5g59040 5'-TCTGTCTCGCCGTCATTTTTGTTAT-3'
At5g46795 5'-GGCTTTGGAGACCAGACTTTTTCAG-3'
At4g26440 5'-TGGAGAGGTAGAAGAGTCCGAATCA-3'
At2g03170 5'-AGAAACACGTCGTTGACGAAGAAAG-3'
At3g14450 5'-GGCCAAGGAGTTTTTCCCTTCTTA-3'
At1g53650 5'-CTCGATCATTGAGCTCAGAAGCTGT-3'.
Construction of promoter::GUS reporters
MSP1-F 5'-AAAAAGCAGGCTTGTCAGTTAGCATGAAAAATTGTATGTTAG-3'
MSP1-R 5'-AGAAAGCTGGGTTTGTTGTGTATACTTGTGTGTGTGTATTTA-3'
MSP2-F 5'-AAAAAGCAGGCTATGTCCTACGATCAGAAGGAGGAG-3'
MSP2-R 5'-AGAAAGCTGGGTAACATGTGATATTATTTTTTTGGTTTATATAGTGG-3'
MSP3-F 5'-AAAAAGCAGGCTTTGTGATATAATAGGTATATATGGTAGAAC-3'
MSP3-R 5'-AGAAAGCTGGGTTGCAAACCCAAGTTTCAGCTTTAAC-3'
  1. Primers used for cDNA synthesis, RT-PCR analyses and construction of promoter::GUS reporters. Where appropriate, appended adapters complementary to AttB1 and AttB2 primers are underlined.