Identification of microspore-active promoters that allow targeted manipulation of gene expression at early stages of microgametogenesis in Arabidopsis

Table 1 Complementation analysis

	% mutant pollen	KmR:KmS T2	pptR:pptS F1	% pptR F1
pMSP1-TIO-3	28.7	48:12	30:61	32.96
pMSP1-TIO-9	14.5	103:5	284:294	49.13
pMSP1-TIO-10	11.9	91:10	193:237	44.88
pMSP1-TIO-18	27.9	56:18	43:90	32.33
pMSP2-TIO-1	40.3	323:67	41:145	22.04
pMSP2-TIO-2	43.3	91:15	29:156	15.68
pMSP2-TIO-5	29.2	88:3	29:60	32.58
pMSP2-TIO-8	46.6	85:31	11:131	7.75
+/tio-3	50.6	-	0:215	0

Four lines harbouring each construct were compared to the heterozygous parent plant, tio-3. The % of mutant pollen was scored after DAPI staining and the T2 segregation of the complementing T-DNA (MSP-TIO) was analysed on kanamycin media. The extent of male transmission (% pptR) of tio-3 was determined on ppt media in F1 progenies from test-crosses.

ISSN: 1471-2229