Skip to main content

Table 1 Complementation analysis

From: Identification of microspore-active promoters that allow targeted manipulation of gene expression at early stages of microgametogenesis in Arabidopsis

  % mutant pollen KmR:KmS
T2
pptR:pptS
F1
% pptR
F1
pMSP1-TIO-3 28.7 48:12 30:61 32.96
pMSP1-TIO-9 14.5 103:5 284:294 49.13
pMSP1-TIO-10 11.9 91:10 193:237 44.88
pMSP1-TIO-18 27.9 56:18 43:90 32.33
pMSP2-TIO-1 40.3 323:67 41:145 22.04
pMSP2-TIO-2 43.3 91:15 29:156 15.68
pMSP2-TIO-5 29.2 88:3 29:60 32.58
pMSP2-TIO-8 46.6 85:31 11:131 7.75
+/tio-3 50.6 - 0:215 0
  1. Four lines harbouring each construct were compared to the heterozygous parent plant, tio-3. The % of mutant pollen was scored after DAPI staining and the T2 segregation of the complementing T-DNA (MSP-TIO) was analysed on kanamycin media. The extent of male transmission (% pptR) of tio-3 was determined on ppt media in F1 progenies from test-crosses.