Micrographs of AVIs in the epidermal cells of fully open lisianthus petals. A. Bright field microscopy image of an unstained transverse section of the inner petal region, showing the distinct morphology of AVIs between the adaxial and abaxial epidermal cells. Irregular AVIs in the adaxial epidermal cells (upper) and vesicle-like AVIs in the abaxial epidermal cells (lower). B. Transverse section of adaxial epidermal cells in Fig. A at higher magnification, showing the central vacuoles (V) and the irregular AVIs (arrowhead). C. Adaxial epidermal peel of the inner petal region under bright field, showing the irregular form of the red-colored AVIs (arrowhead). D. Transverse section of abaxial epidermis of the same inner petal region, showing vesicle-like AVIs (arrow) and central vacuoles (V). E. Abaxial epidermal peel of the inner petal region observed under bright light, showing vesicle-like AVIs (arrow) and central vacuoles.