Skip to main content

Table 1 Comparison of qRT-PCR and microarray results for selected genes.

From: Comprehensive transcriptional profiling of NaCl-stressed Arabidopsis roots reveals novel classes of responsive genes

AGI# annotation microarray (log2 ratio) qRT-PCR (log2 ratio)
   6 h 24 h 48 h 6 h 24 h 48 h
At1g44830 AP2/ERF 4.37 5.42 4.89 6.05 6.37 6.47
At5g43650 bHLH 7.21 7.58 7.45 11.05 11.93 11.87
At4g31500 CYP83B1/SUR2 -2 -1.89 -1.43 -1.84 -1.6 -1.22
At4g26410 expressed protein 0.01 0 0.04 -0.3 -0.23 -0.27
At2g04070 MATE 3.77 5.47 5.35 3.1 4.92 5.27
At3g23250 MYB15 4.05 3.25 1.31 5.39 5.67 5
At4g01550 NAC 1.93 3.35 3.21 2.46 4.87 5.65
At2g37130 PER21 -1.73 -1.56 -0.98 -1.32 -1.4 -0.69
At3g01190 PER27 -0.37 -0.02 0.14 -2.96 -1.21 -0.18
At5g64100 PER69 -1.78 -2.69 -1.8 -1.85 -2.4 -2.2
At2g24570 WRKY17 3.74 1.94 1.16 3.84 2.15 1.64
At2g30250 WRKY25 2.59 4.04 4.49 3.49 6 6.23
At2g38470 WRKY33 4.02 4.42 3.58 4.81 5.58 4.64
At4g30280 XTH18 2.54 1.3 1.57 2.65 1.58 2.5
At3g17860 ZIM 2.28 0.65 -0.84 2.42 1.02 -0.97
  1. Genes representing a variety of expression patterns and functional categories were selected from the microarray dataset for further validation using qRT-PCR. Values shown are the mean of at least four independent measurements (microarray data), or three independent measurements (qRT-PCR).