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Table 1 Side-by-side comparison of three putative PR proteins from apple with their respective type member.

From: PR genes of apple: identification and expression in response to elicitors and inoculation with Erwinia amylovora

  Apple PR-1a PR-1 Type Member CAA29392 Apple PR-5 PR-5 Type Member CAA27548 Apple PR-8 PR-8 Type Member AAC37395
Similarity to Type Member E-value 1 5e-54 N/A 2e-37 N/A 2e-104 N/A
Molecular Weight (Daltons) 17122 18574 25669 24552 31757 30775
Amino Acid Residues 161 168 246 226 299 292
Basic Amino Acids 8 10 14 14 16 13
Acidic Amino Acids 17 14 20 16 24 24
Hydrophobic Amino Acids 55 48 75 63 109 115
Polar Amino Acids 61 54 89 86 102 91
Isoelectric Point 4.718 5.803 4.567 5.248 4.600 4.273
Charge at pH 7.0 -8.296 -3.473 -6.405 -2.435 -7.798 -10.766
Number Amino Acids in Signal Sequence 24 30 24 25 20 25
Predicted Sub-cellular Location Outside Outside Outside Outside Outside Vacuole
  1. 1 The E (expect) value is the probability that the match happened by chance. Comparison was made with mature peptide sequences (i.e. without signal sequence)
  2. Deduced amino acid sequence statistics of PR-1a, PR-5 and PR-8 from apple were generated using the Editseq program in Lasergene® from DNASTAR (Madison, WI, USA). Protein sequences for type members were obtained through GenBank and analyzed using the same program. Sequence similarities to type members were obtained by using the BLAST on the National Center for Biotechnology Information web site. Signal sequence and localization predictions were done by PSORT. The type members are as described by Van Loon et al. [3]