Classification of expressed genes from Mesostigma according to the presence of similar proteins in other organisms in a Venn diagram. All non-redundant expressed genes were used as a query in (t)blastx similarity searches with the Swissprot, Genbank, Chlamydomonas, Cyanidioschyzon, Porphyra, Physcomitrella, Arabidopsis and Oryza data sets. The outermost circle represents all Mesostigma expressed genes. The inner circles, which are labeled chlorophyte, streptophyte and rhodophyte, represent genes, which have similarity to chlorophyte, streptophyte or rhodophyte sequences, respectively. The areas depicted are not proportional to the gene numbers and the number of Mesostigma expressed genes in each category is written in each segment. Numbers in brackets indicate the number of expressed genes in a category after removal of low similarity hits (see Table 2 for a definition of low similarity hits).