Figure 2

Monastrol or STLC treatment induced formation of multipolar spindles with reduced pole-to-pole distance at 24 h AF. (A) Incidence of monasters, multipolar spindles and normal MT arrays with increasing monastrol concentration. Zygotes with normal MT arrays had progressed beyond metaphase at 24 h AF; most were in telophase. (B) Pole-to-pole distance. For multipolar spindles, only the greatest distance between any two spindle poles sharing captured chromosomes was measured. Pole-to-pole distance was significantly (p < 0.001, Student's t test) shorter in all drug treatments compared to controls, and all treatments were significantly different from each other. (C) Multipolar spindles were associated with cytasters. Open bars; percent of the cells with monasters that also had cytasters. Shaded bars; percent of cells with multipolar spindles that also had cytasters.