1. ABRE5
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5'AGCTTGCCGGACACGTGGCGCTCTAG3'
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26 mer
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Annealed with ABRE3 to form 1X ABRE used in EMSA as probe or competitor
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2. ABRE3
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5'CTAGAGCGCCACGTGTCCGGCAAGCT3'
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26 mer
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ABRE probe in EMSA
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3.4X ABRE
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5'CCTCGAAAGCTTGCCACGTGGCGCCACGTGGCGCCACGTGGCGCCACGTGGATGACGCACAATCCCAC3'
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68 mer
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Annealed with 17 LS and filled in by Klenow to form double stranded DNA used as competitor in EMSA
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4.2X ABRC
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5'GTCAGAAAGCTTGCCACGTGGCTGCAGTG CCATTGCCACCGGATCAGCCACGTGGCTCCA GTGCTTGCCACCGGATGACGCACAATCCCAC3'
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92 mer
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Annealed with 17 LS and filled in by Klenow to form double stranded DNA and used as competitor in EMSA
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5. 4X DRE
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5'CCACTTGAAGCTTACTACCGACATGAGTTCACTACCGACATGAGTTCACTACCGACATGACGCACAATCCCAC3'
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72 mer
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Annealed with 17 LS and filled in by Klenow to form double stranded DNA and used as competitor in EMSA.
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6. 2X ERE
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5'AGCAAGCTTTTTTATTCCCAACAATAGAAAGTCTTGTTTTATTCCCAACAATAGAAAGTCTT GATGACGCACAATCCCAC 3'
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80 mer
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Annealed with 17 LS and filled in by Klenow to form double stranded DNA and used as competitor in EMSA
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7. 17 LS
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5'GTGGGATTGTGCGTCAT3'
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17 mer
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Preparation of ds Competitor
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8.OSBZ85
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5'TGTAGATCTCAACAATGGGAAATGACGAAGCTGTAGTTACTCA3'
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43 mer
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Amplification of OSBZ8 cDNA by RT-PCR
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9.OSBZ83
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5'GAGAGATCTTTACTTGAGGCTACAGCATCAGTCGC3'
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36 mer
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Amplification of OSBZ8 cDNA by RT-PCR
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10.OSBZ85A
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5'GGCGTGGAAACTGGTGGAGCT3'
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21 mer
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For sequencing of cDNA
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11.OSBZ83A
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5'CTTGAGAATTCTAAGCTCCACCAGTTTCCACGCC3'
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34 mer
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Amplification of 5'end of OSBZ8
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12. RAB5
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5'CGACAAGCTTCACGCGCACAGTACAC ACACAT-3'
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32 mer
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Amplification of Rab16A promoter and full length by PCR from genomic DNA
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13. RAB3A
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5'TAGAGCTCGGATCCTCTAGACTGCAGT CATGCTGTGGTGTGCAAGCAGG-3'
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49 mer
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Amplification of Rab16A promoter by PCR from genomic DNA
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14. RAB3B
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5'ATAGAGCTCTGGATCCTCAGTGCTGGCCGGGCAGCTT3'
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37 mer
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Amplification of Rab16A full length by PCR from genomic DNA
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