Table 1 Sequence of oligonucleotides used either to prepare double stranded DNA for EMSA or for RT-PCR and PCR:

5'AGCTTGCCGGACACGTGGCGCTCTAG3'

26 mer

Annealed with ABRE3 to form 1X ABRE used in EMSA as probe or competitor

5'CTAGAGCGCCACGTGTCCGGCAAGCT3'

26 mer

ABRE probe in EMSA

5'CCTCGAAAGCTTGCCACGTGGCGCCACGTGGCGCCACGTGGCGCCACGTGGATGACGCACAATCCCAC3'

68 mer

Annealed with 17 LS and filled in by Klenow to form double stranded DNA used as competitor in EMSA

5'GTCAGAAAGCTTGCCACGTGGCTGCAGTG CCATTGCCACCGGATCAGCCACGTGGCTCCA GTGCTTGCCACCGGATGACGCACAATCCCAC3'

92 mer

Annealed with 17 LS and filled in by Klenow to form double stranded DNA and used as competitor in EMSA

5'CCACTTGAAGCTTACTACCGACATGAGTTCACTACCGACATGAGTTCACTACCGACATGACGCACAATCCCAC3'

72 mer

Annealed with 17 LS and filled in by Klenow to form double stranded DNA and used as competitor in EMSA.

5'AGCAAGCTTTTTTATTCCCAACAATAGAAAGTCTTGTTTTATTCCCAACAATAGAAAGTCTT GATGACGCACAATCCCAC 3'

80 mer

Annealed with 17 LS and filled in by Klenow to form double stranded DNA and used as competitor in EMSA

5'GTGGGATTGTGCGTCAT3'

17 mer

Preparation of ds Competitor

5'TGTAGATCTCAACAATGGGAAATGACGAAGCTGTAGTTACTCA3'

43 mer

Amplification of OSBZ8 cDNA by RT-PCR

5'GAGAGATCTTTACTTGAGGCTACAGCATCAGTCGC3'

36 mer

Amplification of OSBZ8 cDNA by RT-PCR

5'GGCGTGGAAACTGGTGGAGCT3'

21 mer

For sequencing of cDNA

5'CTTGAGAATTCTAAGCTCCACCAGTTTCCACGCC3'

34 mer

Amplification of 5'end of OSBZ8

5'CGACAAGCTTCACGCGCACAGTACAC ACACAT-3'

32 mer

Amplification of Rab16A promoter and full length by PCR from genomic DNA

5'TAGAGCTCGGATCCTCTAGACTGCAGT CATGCTGTGGTGTGCAAGCAGG-3'

49 mer

Amplification of Rab16A promoter by PCR from genomic DNA

5'ATAGAGCTCTGGATCCTCAGTGCTGGCCGGGCAGCTT3'

37 mer

Amplification of Rab16A full length by PCR from genomic DNA