Table 1 HPLC analysis of tuber carotenoids (ng/g dry weight). Carotenoids were measured via diode array HPLC (see Methods) on a minimum of 4 different tubers from 2 different plants. Fold variation with respect to the wild-type is reported for each carotenoid compound and for each line.

Wild-type

43.07± 12.78

588.01± 108.14

3.17± 1.01

262.64± 108.08

1301.33± 479.34

1109.08± 420.06

541.02± 98.14

89.34± 13.02

734.35± 173.04

4672.02± 1414.62

Gus 1

52.01± 5.87

531.46± 153.57

3.76± 1.32

223.02± 63.04

1091.14± 293.24

1157.69± 332.49

685.32± 204.95

81.34± 22.40

1270.39± 299.42

5096.15± 1376.25

Fold Variation

1.21

0.90

1.20

0.85

0.84

1.04

1.27

0.91

1.73

1.090

AS-e 1

160.59± 8.03

725.46± 100.33

17.17± 3.59

626.28± 187.37

2173.42± 97.33

2788.91± 170.82

303.66± 83.88

110.94± 38.43

2015.11± 525.48

8921.56± 1218.27

Fold Variation

3.73

1.23

5.42

2.38

1.67

2.51

0.56

1.24

2.74

1.909

AS-e 2

248.59± 62.92

506.12± 112.22

14.35± 3.14

669.39± 50.98

3045.66± 493.89

3320.07± 1061.82

1219.54± 20.21

158.88± 79.35

3089.75± 69.24

12272.36± 1961.77

Fold Variation

5.77

0.86

4.53

2.55

2.34

2.99

2.25

1.78

4.21

2.627

AS-e 3

86.97± 6.34

866.91± 104.46

9.48± 1.84

465.48± 146.89

1703.35± 150.12

2917.70± 720.57

945.84± 318.98

125.80± 55.78

1555.41± 589.078

8677.12± 2094.07

Fold Variation

2.02

1.47

2.99

1.77

1.31

2.63

1.75

1.41

2.19

1.857

AS-e 4

193.70± 1.23

1044.59± 144.40

43.56± 14.24

990.83± 197.51

2198.54± 559.12

2935.03± 797.13

612.56± 245.85

90.14± 24.62

1865.33± 1010.02

9974.29± 2994.11

Fold Variation

4.50

1.78

13.74

3.77

1.70

2.65

1.13

1.01

2.54

2.135

AS-e 5

35.44± 5.22

510.42± 99.42

2.60± 0.92

324.83± 111.81

1266.71± 78.11

1124.44± 409.81

421.81± 173.67

98.73± 20.30

776.25± 87.11

4561.23± 986.38

Fold Variation

0.82

0.87

0.82

1.24

0.97

1.01

0.78

1.10

1.06

0.977

AS-e 6

50.21± 8.87

469.53± 137.01

3.32± 2.01

338.81± 87.43

1369.53± 263.04

1378.81± 359.37

746.48± 221.87

76.81± 13.37

688.91± 118.44

5123.42± 1211.42

Fold Variation

1.17

0.80

1.05

1.29

1.05

1.24

1.38

0.86

0.99

1.097