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Table 1 HPLC analysis of tuber carotenoids (ng/g dry weight). Carotenoids were measured via diode array HPLC (see Methods) on a minimum of 4 different tubers from 2 different plants. Fold variation with respect to the wild-type is reported for each carotenoid compound and for each line.

From: Metabolic engineering of potato tuber carotenoids through tuber-specific silencing of lycopene epsilon cyclase

Line Phytofluene Lutein β-carotene Zeaxanthin Antheraxanthin Violaxanthin Neoxanthin Other Xanthophylls Esters Total Carotenoids
Wild-type 43.07± 12.78 588.01± 108.14 3.17± 1.01 262.64± 108.08 1301.33± 479.34 1109.08± 420.06 541.02± 98.14 89.34± 13.02 734.35± 173.04 4672.02± 1414.62
Gus 1 52.01± 5.87 531.46± 153.57 3.76± 1.32 223.02± 63.04 1091.14± 293.24 1157.69± 332.49 685.32± 204.95 81.34± 22.40 1270.39± 299.42 5096.15± 1376.25
Fold Variation 1.21 0.90 1.20 0.85 0.84 1.04 1.27 0.91 1.73 1.090
AS-e 1 160.59± 8.03 725.46± 100.33 17.17± 3.59 626.28± 187.37 2173.42± 97.33 2788.91± 170.82 303.66± 83.88 110.94± 38.43 2015.11± 525.48 8921.56± 1218.27
Fold Variation 3.73 1.23 5.42 2.38 1.67 2.51 0.56 1.24 2.74 1.909
AS-e 2 248.59± 62.92 506.12± 112.22 14.35± 3.14 669.39± 50.98 3045.66± 493.89 3320.07± 1061.82 1219.54± 20.21 158.88± 79.35 3089.75± 69.24 12272.36± 1961.77
Fold Variation 5.77 0.86 4.53 2.55 2.34 2.99 2.25 1.78 4.21 2.627
AS-e 3 86.97± 6.34 866.91± 104.46 9.48± 1.84 465.48± 146.89 1703.35± 150.12 2917.70± 720.57 945.84± 318.98 125.80± 55.78 1555.41± 589.078 8677.12± 2094.07
Fold Variation 2.02 1.47 2.99 1.77 1.31 2.63 1.75 1.41 2.19 1.857
AS-e 4 193.70± 1.23 1044.59± 144.40 43.56± 14.24 990.83± 197.51 2198.54± 559.12 2935.03± 797.13 612.56± 245.85 90.14± 24.62 1865.33± 1010.02 9974.29± 2994.11
Fold Variation 4.50 1.78 13.74 3.77 1.70 2.65 1.13 1.01 2.54 2.135
AS-e 5 35.44± 5.22 510.42± 99.42 2.60± 0.92 324.83± 111.81 1266.71± 78.11 1124.44± 409.81 421.81± 173.67 98.73± 20.30 776.25± 87.11 4561.23± 986.38
Fold Variation 0.82 0.87 0.82 1.24 0.97 1.01 0.78 1.10 1.06 0.977
AS-e 6 50.21± 8.87 469.53± 137.01 3.32± 2.01 338.81± 87.43 1369.53± 263.04 1378.81± 359.37 746.48± 221.87 76.81± 13.37 688.91± 118.44 5123.42± 1211.42
Fold Variation 1.17 0.80 1.05 1.29 1.05 1.24 1.38 0.86 0.99 1.097