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Table 3 Quantitative real-time PCR analysis of mRNA for Gal4 and LUC genes in selected linesa.

From: Development and evaluation of a Gal4-mediated LUC/GFP/GUS enhancer trap system in Arabidopsis

Line# Fold drop in LUC activity (generations used)b Fold drop in gene expression
   GAL4 LUC
169 1.2 (T3/T4) 3 3.5
213 1.8(T3/T4) 2.3 3.3
65L-4B 42 (T2/T4) 1.6 147
50-1 33 (T2/T4) 59 56
36 c 82 (T2/T4) 298 71
80-2 20 (T2/T4) 18 35
  1. a All numbers indicate a fold decrease in activity or expression as plant lines progress from the T2 (or T3) to the T4 generation.
  2. b Luciferase activity measured in BASTA-selected 14 day-old seedlings by spraying 1 mM luciferin.
  3. c This is a full 35S-driven Gal4 line as shown in Fig 8.