Development and evaluation of a Gal4-mediated LUC/GFP/GUS enhancer trap system in Arabidopsis

BMC Plant Biology

Table 3 Quantitative real-time PCR analysis of mRNA for Gal4 and LUC genes in selected lines^a.

Line#	Fold drop in LUC activity (generations used)^b	Fold drop in gene expression
		GAL4	LUC
169	1.2 (T3/T4)	3	3.5
213	1.8(T3/T4)	2.3	3.3
65L-4B	42 (T2/T4)	1.6	147
50-1	33 (T2/T4)	59	56
36 ^c	82 (T2/T4)	298	71
80-2	20 (T2/T4)	18	35

^a All numbers indicate a fold decrease in activity or expression as plant lines progress from the T2 (or T3) to the T4 generation.
^b Luciferase activity measured in BASTA-selected 14 day-old seedlings by spraying 1 mM luciferin.
^c This is a full 35S-driven Gal4 line as shown in Fig 8.

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