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Table 2 Comparison of luminescence across generations for randomly selected trap lines.

From: Development and evaluation of a Gal4-mediated LUC/GFP/GUS enhancer trap system in Arabidopsis

Line # T2a T3 T4 Fold Change Fold Change Fold Change
     T2 to T3 T3 to T4 T2 to T4
172 6522 6145 5609 1.1 Same 1.1 Same 1.2 Same
216 2354 2485 2589 0.9 Same 1.0 Same 0.9 Same
25-1 16170 4990 51479 3.2 Decrease 10.3 Increase 3.2 Increase
169 12007 34529 24193 2.9 Increase 1.4 Decrease 2.0 Increase
173 4524 6452 6678 1.4 Increase 1.0 Same 1.5 Increase
212 5157 4150 3318 1.2 Same 1.3 Same 1.6 Decrease
27-4 9859 4283 5129 2.3 Decrease 1.2 Same 1.9 Decrease
47-1 6531 2680 3224 2.4 Decrease 1.2 Same 2.0 Decrease
14-1 3914 4110 1733 1.0 Same 2.4 Decrease 2.3 Decrease
213 2434 5286 895 2.2 Increase 5.9 Decrease 2.7 Decrease
214 3933 2171 1028 1.8 Decrease 2.1 Decrease 3.8 Decrease
211 8475 2423 1251 3.5 Decrease 1.9 Decrease 6.8 Decrease
77-2 1872 3668 200 2.0 Increase 18.0 Decrease 9.4 Decrease
215 11211 5995 479 1.9 Decrease 12.5 Decrease 23.4 Decrease
65L-4B 16022 2987 0 5.4 Decrease 3.0 × 103 Decrease 1.6 × 104 Decrease
18-1 1968 1163 0 1.7 Decrease 1.0 × 103 Decrease 2.0 × 103 Decrease
80-2 12378 6187 19 2.0 Decrease 3.0 × 102 Decrease 6.0 × 102 Decrease
50-1 16746 3867 19 4.3 Decrease 2.0 × 102 Decrease 8.0 × 102 Decrease
  1. a. Luminescence was quantified for pRGK335 (trap) plant lines in multiple generations. Individual 14 day-old seedlings from each generation were grown on the same plates and whole plant luminescence quantified. Luminescence is expressed as Actual minus Background per mm2. 100 μM luciferin was included in the media. For the T2 lines, data is an average of 3 to 5 plants; only luminescent plants were included in the data (As described in the text, luminescence segregated/correlated with BASTA-resistance). For the T3 and T4 generations, all seedlings were imaged, as all were correlated with BASTA-resistance.