Development and evaluation of a Gal4-mediated LUC/GFP/GUS enhancer trap system in Arabidopsis

BMC Plant Biology

Table 2 Comparison of luminescence across generations for randomly selected trap lines.

Line #	T2^a	T3	T4	Fold Change		Fold Change		Fold Change
				T2 to T3		T3 to T4		T2 to T4
172	6522	6145	5609	1.1	Same	1.1	Same	1.2	Same
216	2354	2485	2589	0.9	Same	1.0	Same	0.9	Same
25-1	16170	4990	51479	3.2	Decrease	10.3	Increase	3.2	Increase
169	12007	34529	24193	2.9	Increase	1.4	Decrease	2.0	Increase
173	4524	6452	6678	1.4	Increase	1.0	Same	1.5	Increase
212	5157	4150	3318	1.2	Same	1.3	Same	1.6	Decrease
27-4	9859	4283	5129	2.3	Decrease	1.2	Same	1.9	Decrease
47-1	6531	2680	3224	2.4	Decrease	1.2	Same	2.0	Decrease
14-1	3914	4110	1733	1.0	Same	2.4	Decrease	2.3	Decrease
213	2434	5286	895	2.2	Increase	5.9	Decrease	2.7	Decrease
214	3933	2171	1028	1.8	Decrease	2.1	Decrease	3.8	Decrease
211	8475	2423	1251	3.5	Decrease	1.9	Decrease	6.8	Decrease
77-2	1872	3668	200	2.0	Increase	18.0	Decrease	9.4	Decrease
215	11211	5995	479	1.9	Decrease	12.5	Decrease	23.4	Decrease
65L-4B	16022	2987	0	5.4	Decrease	3.0 × 10³	Decrease	1.6 × 10⁴	Decrease
18-1	1968	1163	0	1.7	Decrease	1.0 × 10³	Decrease	2.0 × 10³	Decrease
80-2	12378	6187	19	2.0	Decrease	3.0 × 10²	Decrease	6.0 × 10²	Decrease
50-1	16746	3867	19	4.3	Decrease	2.0 × 10²	Decrease	8.0 × 10²	Decrease

a. Luminescence was quantified for pRGK335 (trap) plant lines in multiple generations. Individual 14 day-old seedlings from each generation were grown on the same plates and whole plant luminescence quantified. Luminescence is expressed as Actual minus Background per mm². 100 μM luciferin was included in the media. For the T2 lines, data is an average of 3 to 5 plants; only luminescent plants were included in the data (As described in the text, luminescence segregated/correlated with BASTA-resistance). For the T3 and T4 generations, all seedlings were imaged, as all were correlated with BASTA-resistance.

ISSN: 1471-2229