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Table 1 GUS expression in 1X, 2X, and 5X UAS/GFP-GUS plants with different Gal4 backgrounds.

From: Development and evaluation of a Gal4-mediated LUC/GFP/GUS enhancer trap system in Arabidopsis

No Gal4

 

1X

2X

5X

# of Plants a

6

11

8

Gus Expression:

   

None

2

6

4

Low

4

4

4

High

0

1

0

m35S/Gal4

 

1X

2X

5X

# of Plants

14

15

17

Gus Expression:

   

None

8

8

6

Low

3

4

6

High

3

3

5

e35S/Gal4

 

1X

2X

5X

# of Plants

26

15

22

Gus Expression:

   

None

12

9

2

Low

7

4

7

High

7

2

13

  1. a T1 seedlings were intially selected on the appropriate chemicals (BASTA and/or hygromycin) and transplanted to soil. Two leaves from each plant were collected after two weeks in soil for Gus assay (as shown in Figure 7).