Maps of plasmids and selected DNA sequences of constructs. A, pRGK336 has enhanced 35S upstream of Gal4 while pRGK335 has minimal 35S upstream of Gal4. In both constructs, 5X UAS and m35S are upstream of the luciferase gene (psp-luc). B, There is no Gal4 in pRGK337and the 5XUAS and m35S are upstream of the GFP-GUS fusion protein. 2XUAS and 1XUAS versions are the same map but with the sequence changes noted in Fig 1C and the text. C, Selected DNA sequences of indicated regions. The right border is underlined and the TATA box is boxed; m35S and e35S are in bold. The ATG start site is bolded and underlined with an arrow for the indicated orfs. The UAS sequences are in bold and underlined showing the difference between 1X, 2X and 5X UAS.