Floral gene resources from basal angiosperms for comparative genomics research

BMC Plant Biology

Table 1 Current (12/01/04) statistics for Floral Genome Project cDNA libraries, EST sequences and unigene builds. We will perform 10,000 EST sequencing reactions for each taxon listed in the top portion of the table and 2000 ESTs for each taxon in the bottom portion of the table.

Taxon	Primary Titre (pfu)	Amplified Titre (pfu/ml)	Avg Insert size (bp)	ESTs to date	^aUnigenes	^bObserved Redundancy
Libraries for deep sequencing
Welwitschia mirabilis (m) – Gymnosperm	3.25E+05	6.00E+08	1382	3732	2771	34.7%
Amborella trichopoda (m) – Basal angiosperm	2.24E+06	1.37E+10	1611	4047	6099	41.5%
Amborella trichopoda (f)	4.98E+06	1.40E+10	1031	4442
Nuphar advena (Water lily, Spadderdock) – Basal angiosperm	2.00E+06	3.20E+10	1134	8442	6205	36.1%
Acorus americanus (Sweet flag) – Basal monocot.	2.80E+06	6.00E+09	1083	5883	3067	28.8%
^cAsparagus officinalis (m) – Transformable nongrass monocot	1.50E+06	1.20E+10	1468	5188	4560	61.5%
Asparagus officinalis (f)	1.30E+06	1.40E+10	1200	2174
Persea americana (Avocado) – Cultivated magnoliid	2.74E+06	2.57E+10	1349	8735	5314	41.3%
Liriodendron tulipifera (Tulip Poplar, Yellow Poplar) – Transformable lumber species	3.00E+06	2.00E+10	1346	9531	6520	46.2%
Saruma henryi (Upright Wild Ginger) – member of Aristolochiaceae with bipartite perianth	1.97E+06	1.67E+10	1587	3230	2631	21.8%
Eschscholzia californica (California poppy) – Transformable basal eudicot	7.00E+06	1.68E+11	1702	9079	6015	46.18%
Libraries for shallow sequencing
^dCucumis sativus (m) (Cucumber) – rosid	------	------	------	1107	1648	23.5%
^d Cucumis sativus (f)	------	------	------	928
Ribes americanum (Black currant)	2.58E+06	2.25E+10	1200	2238	1791	25.0%
^eVaccinium corymbosum (Blueberry) – basal asterid	------	------	------	1758	1549	13.5%
			Total:	70,514	48,170

m indicates library constructed from male tissues; f for female tissues.
^aUnigene numbers are shown in the first line for taxa with multiple libraries.
^bObserved redundancy was measured for each taxon as (EST# – Unigene #)/Unigene#.
^c5188 ESTs from the male Asparagus library were sequenced in collaboration with Mike Havey (University of Wisconsin) and Chris Town (TIGR).
^dMale and female Cucumis flower bud libraries described by Perl-Treves et al [71]
^eVaccinium young inflorescence library was provided by Jeannine Rowland (USDA) [72].

ISSN: 1471-2229