Figure 5

Features and properties of enzymatically isolated DNA. A: comparison of the amount of DNA isolated from Ilex aquifolium leaves by a conventional extraction method (lines 1–5) and by the enzymatic method described here (lines 6–10). In both case the amount of loaded DNA is one fifth of the DNA corresponding to one leaf disk. B: Study of the stability of lambda DNA/Hind III during the digestion of leaf disks of Ilex. Line 1: one leaf disk alone; line 2: one leaf disk and lambda DNA/Hind III; line 3: lambda DNA/Hind III alone. C, D, E, and F: PCR amplification of enzymatically isolated DNA from Ilex aquifolium (line 1), Aster amellus (line 2), and Solanum tuberosum (line 3). C: PCR amplification of the plastid atpB-rbcL spacer. D: PCR amplification of ITS/5.8S. E: PCR amplification of the nuclear encoded plastid glutamine synthetase. F: RAPD amplification.