Figure 1

Electrophoretic aspect of enzymatically isolated DNA. A: Agarose gel electrophoresis of typical enzymatically isolated DNA from 24 different species (in the following order: Phlomis fructicosa, Humulus lupulus, Veratrum album, Scilla bifolia, Astragalus gummifer, Vitis vinifera, Centaurea macrocephala, Narcissus pseudonarcissus, Allium ampeloprassum, Salvia officinalis, Viburnum carlesii, Colchicum speciosum, Triticum turgidum, Polygonum chinensis, Lathyrus vernus, Tilia sp., Caragana sophorifolia, Urtica dioica, Lilium henryi, Polygonum multiflorus, Geranium sp., Lupinus sp., Crocus albiflorus, Helleborus dumetorum). After digestion, the DNA was isolated with Dynabeads^® DNA DIRECT™ Universal magnetic beads. One fourth of the isolated DNA was loaded. The first and last lines were loaded with 250 ng of lambda/HindIII DNA (500 ng, bottom half, right). B: Agarose gel electrophoresis of DNA of 42 randomly chosen species (in the following order: Danae racemosa, Epimedium alpinum, Gladiolus palustris, Viburnum farreri, Euonymus bungeana, Weigela sp., Prunus padus, Rhodea japonica, Polygonatum multiflorum, Daphne japonica, Ribes petraeum, Asplenuim scolopendrium, Carex morrowii, Aruncus dioicus, Bletilla striata, Helleborus odoratus, Hedera helix, Brunnera macrophylla, Paeonia belladonna, Atropa belladonna, Solanum tuberosum, Beta vulgaris, Anethum graveolens, Allium fistulosum, Sison amomum, Uniola latifolia, Sinningia magnifica, Peperomia sp., Alnus sp., Tillia sp., Betula sp., Skimmia sp., Liriope spicata, Anthericum liliago, Inula ensifolia, Phlomis fruticosa, Lilium pumilum, Sorbaria sorbifolia, Dietes bicolor, Ilex aquifolium, Vitis vinifera, Setaria italica, Triticum aestivum, Nymphea sp., Pelargonium sp., Saintpaulia magungensis, Morinda sp., Zea mais). After enzymatic digestion in half of a 96 microtitration plate, DNA was isolated using Wizard^® Magnetic 96 Plant System magnetic beads. One fourth of the isolated DNA was loaded.