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Table 2 Genotypes at the VMC 5g7 and VMC 6c10 loci of clones regenerated through embryogenesisor shoot organogenesis

From: Genetic chimerism of Vitis viniferacv. Chardonnay 96 is maintained through organogenesis but not somatic embryogenesis

 

Locus alleles (bp)

Plant origin

DNA origin

VMC 6c10

VMC 5g7

cell layer of origin

anthers

    

   Inflorescence A

    

   Primary (20) or secondary (6) somaclones

Leaves iv + gh

114:140

198:222

L1

 

Rootlets iv

114:140

198:222

L1

   Inflorescence B

    

   Primary somaclones (3)

Leaves iv

114:140

198:222

L1

   Inflorescence C

    

   Primary (5) or secondary (1) somaclones

Leaves iv + gh

114:140

198:222

L1

   Inflorescence D

    

   Primary somaclone (1)

Leaves iv + gh

114:140

198:222

L1

nodal explants

    

   Primary somaclones (16)

    
 

Leaves iv

114:140

198:222

L1

 

Rootlets iv

114:140

198:222

L1

meristematic bulk

    

   clones (7)

    
 

Leaves iv

114:140:142

198:220:222

L1 + L2

  1. from:
  2. – anthers from 4 different inflorescences (A, B, C, D)
  3. – nodal explants
  4. or through shoot organogenesis from meristematic bulks
  5. (N°) : number of clones, iv : in vitro, gh : greenhouse