Figure 4

AML mutant allele characterization. i) PCR product to identify the wild-type allele of each AML locus. ii) PCR product to identify the mutant allele of each AML locus. iii) PCR product to assay for accumulation of cDNA from transcripts transcribed across the insertion site. iv) PCR product to assay for accumulation of cDNA from transcripts transcribed from the AML locus downstream of the insertion site. AML4 was excluded from this assay because the mutant allele is disrupted in the center of its coding region. Q6 refers to genomic DNA or cDNA template extracted from quintuple insertion allele homozygotes; Ws refers to genomic DNA or cDNA template extracted from wild-type (Wassilijewskia ecotype) plants.