Figure 1

AML1 interacts with AtRaptor1B in a yeast two-hybrid assay. A. Schematic diagram of AML1 fragments cloned into pGADT7. The AML1 RNA Recognition Motifs (RRMs) are indicated. Fragments interacting with AtRaptor1B are labeled in red. B. Yeast two-hybrid results. Numbers on each plate refer to the pGAD construct in A. For each plate, the set of six cultures on the top half were co-transformed with pGBK:Raptor; the set of six cultures on the bottom half were co-transformed with pGBKT7 vector. The plate at left lacks leucine, tryptophan, histidine and adenine and selects for a protein-protein interaction. The control plate at right lacks leucine and tryptophan, and indicates co-transformation of the yeast cells with both a pGAD and a pGBK plasmid.