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Table 2 Purification of AtGA3OX4 from E. coli cells (approx. 7 g)

From: Purification and kinetic studies of recombinant gibberellin dioxygenases

Purification step

Protein (mg)

Specific activity (pkat/mg)

Total activity (pkat)

Apparent purification Factor

Yield (%)

Soluble cell fraction

790

360

284,400

-

-

IMAC chromatography

4.0

4,325

17,300

12X

6%

Superdex 75

0.075

5,466

410

1.26X

0.14%