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Table 2 Purification of AtGA3OX4 from E. coli cells (approx. 7 g)

From: Purification and kinetic studies of recombinant gibberellin dioxygenases

Purification step Protein (mg) Specific activity (pkat/mg) Total activity (pkat) Apparent purification Factor Yield (%)
Soluble cell fraction 790 360 284,400 - -
IMAC chromatography 4.0 4,325 17,300 12X 6%
Superdex 75 0.075 5,466 410 1.26X 0.14%