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Table 1 Purification of PsGA2OX1 from E. coli cells (approx. 6 g)

From: Purification and kinetic studies of recombinant gibberellin dioxygenases

Purification step Protein (mg) Specific activity* (pkat/mg) Total activity (pkat)* Apparent purification factor* Yield*
Soluble cell fraction 670 0.013 8.71 - -
Affinity chromatography (after GST removal) 3.8 0.320 1.2 24X 14%
Superdex 75 1.8 0.89 1.6 2.8X 18%
  1. *Figures influenced by EDTA of buffers in the first two steps