Table 1 Purification of PsGA2OX1 from E. coli cells (approx. 6 g)
From: Purification and kinetic studies of recombinant gibberellin dioxygenases
Purification step | Protein (mg) | Specific activity* (pkat/mg) | Total activity (pkat)* | Apparent purification factor* | Yield* |
|---|---|---|---|---|---|
Soluble cell fraction | 670 | 0.013 | 8.71 | - | - |
Affinity chromatography (after GST removal) | 3.8 | 0.320 | 1.2 | 24X | 14% |
Superdex 75 | 1.8 | 0.89 | 1.6 | 2.8X | 18% |