Figure 2
From: Purification and kinetic studies of recombinant gibberellin dioxygenases
Purification of recombinant PsGA2OX1 shown by SDS-PAGE on a 12% gel with Coomassie Blue staining. (A) Lanes: 1, soluble cell fraction; 2, GST-PsGA2OX1 after affinity chromatography; 3, PSGA2OX1 after thrombin digest and GST removal; 4, thrombin digest of GST-PsGA2OX1 with GST present; 5, Bio Rad molecular weight standards, (from top) 93, 49.8, 35.8, 29.2 and 21.3 kDa. (B) Lanes: 1, concentrated GST-PsGA2OX1 (uncleaved, 10 mg/mL); 2, concentrated PsGA2OX1 (10 mg/mL) after gel filtration on Superdex 75. The molecular weights expected for GST-PsGA2OX1, PsGA2OX1 and GST are 62.8, 36.8 and 26 kDa, respectively. The arrows indicate the position of PsGA2OX1.