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Table 3 Permutations of PCR component concentrations used for optimization of PCR conditions for each marker.

From: Development of ESTs from chickpea roots and their use in diversity analysis of the Cicergenus

Reactions Primer (pmol) Template (ng) MgCl2 (mM) dNTP (mM) Enzyme (u)
1 0.2 5 1 0.1 0.2
2 0.2 15 2 1.5 0.5
3 0.3 5 1.5 0.2 0.5
4 0.3 10 2 0.1 0.3
5 0.5 10 1 0.2 0.3