Table 3 Permutations of PCR component concentrations used for optimization of PCR conditions for each marker.
From: Development of ESTs from chickpea roots and their use in diversity analysis of the Cicergenus
Reactions | Primer (pmol) | Template (ng) | MgCl2 (mM) | dNTP (mM) | Enzyme (u) |
|---|---|---|---|---|---|
1 | 0.2 | 5 | 1 | 0.1 | 0.2 |
2 | 0.2 | 15 | 2 | 1.5 | 0.5 |
3 | 0.3 | 5 | 1.5 | 0.2 | 0.5 |
4 | 0.3 | 10 | 2 | 0.1 | 0.3 |
5 | 0.5 | 10 | 1 | 0.2 | 0.3 |