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Table 5 Characteristics of chickpea EST markers including SSR motif where present, annotation information (NSH indicates no significant homology), and, size range of amplification products, number of alleles and PIC (polymorphism information content) values based on screening of germplasm in Table 1.

From: Development of ESTs from chickpea roots and their use in diversity analysis of the Cicergenus

Marker SSR motif Annotation Product size (bp) No. of alleles PIC
AGLC2   Valyl tRNA synthetase 620 2 0.50
AGLC7   D-3-phosphoglycerate dehydrogenase 330–335 2 0.44
AGLC8   RNA helicase 525–550 5 0.78
AGLC9   Unknown protein 680 4 0.62
AGLC14   Hypothetical protein 520–600 2 0.45
AGLC15   Hypothetical protein 520–600 4 0.65
AGLC16   WD-repeat protein like 365–370 2 0.32
AGLC19   Formin-like protein 350–355 4 0.50
AGLC20   Type IIB calcium ATPase 230–235 3 0.61
AGLC27   Putative Scarecrow gene regulator 330–335 3 0.43
AGLC28   Type 2A protein phosphatase 335–350 3 0.86
AGLC29   Non-specific lipid transfer protein 325–400 6 0.64
AGLC34 AG(20) Putative protein kinase 350–600 5 0.75
AGLC39 AG(17) No significant homology 275–290 3 0.74
AGLC45   Probable cysteine proteinase (EC 3.4.22.-) 410–420 2 0.29
AGLC47   (NM_127184) putative beta-ketoacyl-CoA synthase 200–210 2 0.59
AGLC48   Putative peroxisome assembly factor-2 (NM_100181) 400–410 3 0.89
AGLC51   RNA binding protein 170–180 4 0.55
AGLC52   Protein kinase 390–410 6 0.89
AGLC53   Acyl- [acyl-carrier protein] desaturase 390–400 5 0.87
AGLC55 CT(17) Putative protein kinase 225–275 4 0.89
AGLC57 GGA(3) Unknown protein; protein >1000 3 0.56
AGLC60 TTC(3) Hypothetical protein 310–350 7 0.82
AGLC61 GAA(3) Protein of unknown function 450–470 5 0.55
AGLC64 TTC(6) No significant homolgy 270–360 4 0.20
AGLC66   Probable cysteine protenase (EC 3.4.22.-) 500–650 8 0.63
AGLC67 TCT(4) Tryptophan synthase beta-subunit (TSB2) >1000 4 0.56
AGLC68   Lon protease homolog 1 precursor 220–230 3 0.39
AGLC72   Putative Grr1 protein 230–250 4 0.62
AGLC74 TTTA(3) Glycosyl hydrolase family 17 400–450 5 0.75
AGLC75 AAC(5) No significant homology 200–230 3 0.48
AGLC76 AAG(3) RGA2 protein Arabidopsis thaliana 440–480 3 0.71
AGLC78 CAA(5) Translation initiation factor eIF3 – like protein 430–450 2 0.44
AGLC79   No significant homology 330–410 5 0.03
AGLC82   Unknown protein; protein id: At1g80280.1 300–320 3 0.50
AGLC83   Putative valyl tRNA synthetase [Oryza sativa-japonica cultivar-group] >1000 3 0.74
AGLC84 AAG(3) DEAD/DEAH box RNA helicase 430–450 3 0.42
AGLC85   Emb trypsin inhibitor cme precursor 410–450 3 0.47
AGLC88   Chromomethylase CMT2 Arabidopsis thaliana >1000 5 0.78
AGLC93   BZIP family transcription factor >1000 2 0.51
AGLC94   T48 ankyrin-like protein 480–500 4 0.67
AGLC96   Hypothetical protein CAB95829.1 310–360 5 0.59
AGLC98 AG(19) Hypothetical protein 405–450 5 0.78
AGLC101   No Significant homology 370–400 4 0.70