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Table 5 Characteristics of chickpea EST markers including SSR motif where present, annotation information (NSH indicates no significant homology), and, size range of amplification products, number of alleles and PIC (polymorphism information content) values based on screening of germplasm in Table 1.

From: Development of ESTs from chickpea roots and their use in diversity analysis of the Cicergenus

Marker

SSR motif

Annotation

Product size (bp)

No. of alleles

PIC

AGLC2

 

Valyl tRNA synthetase

620

2

0.50

AGLC7

 

D-3-phosphoglycerate dehydrogenase

330–335

2

0.44

AGLC8

 

RNA helicase

525–550

5

0.78

AGLC9

 

Unknown protein

680

4

0.62

AGLC14

 

Hypothetical protein

520–600

2

0.45

AGLC15

 

Hypothetical protein

520–600

4

0.65

AGLC16

 

WD-repeat protein like

365–370

2

0.32

AGLC19

 

Formin-like protein

350–355

4

0.50

AGLC20

 

Type IIB calcium ATPase

230–235

3

0.61

AGLC27

 

Putative Scarecrow gene regulator

330–335

3

0.43

AGLC28

 

Type 2A protein phosphatase

335–350

3

0.86

AGLC29

 

Non-specific lipid transfer protein

325–400

6

0.64

AGLC34

AG(20)

Putative protein kinase

350–600

5

0.75

AGLC39

AG(17)

No significant homology

275–290

3

0.74

AGLC45

 

Probable cysteine proteinase (EC 3.4.22.-)

410–420

2

0.29

AGLC47

 

(NM_127184) putative beta-ketoacyl-CoA synthase

200–210

2

0.59

AGLC48

 

Putative peroxisome assembly factor-2 (NM_100181)

400–410

3

0.89

AGLC51

 

RNA binding protein

170–180

4

0.55

AGLC52

 

Protein kinase

390–410

6

0.89

AGLC53

 

Acyl- [acyl-carrier protein] desaturase

390–400

5

0.87

AGLC55

CT(17)

Putative protein kinase

225–275

4

0.89

AGLC57

GGA(3)

Unknown protein; protein

>1000

3

0.56

AGLC60

TTC(3)

Hypothetical protein

310–350

7

0.82

AGLC61

GAA(3)

Protein of unknown function

450–470

5

0.55

AGLC64

TTC(6)

No significant homolgy

270–360

4

0.20

AGLC66

 

Probable cysteine protenase (EC 3.4.22.-)

500–650

8

0.63

AGLC67

TCT(4)

Tryptophan synthase beta-subunit (TSB2)

>1000

4

0.56

AGLC68

 

Lon protease homolog 1 precursor

220–230

3

0.39

AGLC72

 

Putative Grr1 protein

230–250

4

0.62

AGLC74

TTTA(3)

Glycosyl hydrolase family 17

400–450

5

0.75

AGLC75

AAC(5)

No significant homology

200–230

3

0.48

AGLC76

AAG(3)

RGA2 protein Arabidopsis thaliana

440–480

3

0.71

AGLC78

CAA(5)

Translation initiation factor eIF3 – like protein

430–450

2

0.44

AGLC79

 

No significant homology

330–410

5

0.03

AGLC82

 

Unknown protein; protein id: At1g80280.1

300–320

3

0.50

AGLC83

 

Putative valyl tRNA synthetase [Oryza sativa-japonica cultivar-group]

>1000

3

0.74

AGLC84

AAG(3)

DEAD/DEAH box RNA helicase

430–450

3

0.42

AGLC85

 

Emb trypsin inhibitor cme precursor

410–450

3

0.47

AGLC88

 

Chromomethylase CMT2 Arabidopsis thaliana

>1000

5

0.78

AGLC93

 

BZIP family transcription factor

>1000

2

0.51

AGLC94

 

T48 ankyrin-like protein

480–500

4

0.67

AGLC96

 

Hypothetical protein CAB95829.1

310–360

5

0.59

AGLC98

AG(19)

Hypothetical protein

405–450

5

0.78

AGLC101

 

No Significant homology

370–400

4

0.70