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Table 2 Characteristics of chickpea EST markers developed from EST root library with primer sequence, GenBank accession numbers, PCR optimized parameters including touch down amplification profile.

From: Development of ESTs from chickpea roots and their use in diversity analysis of the Cicergenus

Marker

Primer Sequence (5'--3') Forward

Primer Sequence (5'--3') Reverse

GenBank accession numbers

Touch -down profile (°C)

PCR optimised reagents

     

Primer (pmol)

Mg++ (mM)

dNTP (mM)

Taq (u)

AGLC2

TGTCAGACTGAGCTGTGTATGAGA

TTGCCCGTATGGTTATGTTAGGAA

CK148953*

55–45

0.3

1.5

0.15

0.3

AGLC7

GACCCCCAAAAATGAAAAAGCA

TTGCCCATACATTCTTCACCCAA

CK148966

60–55

0.3

1.5

0.15

0.3

AGLC8

CAAACTCCTCAATAGCAGGCACA

GCTGTATCGGAGAGTGGTCAGA

CK149041*

55–45

0.3

1.5

0.15

0.3

AGLC9

ACTCCTGTAGTGGCATATCTTCGA

TGGTCCATTTATGCCGCTGGTA

CK148709

60–55

0.3

1.5

0.15

0.3

AGLC14

GCAGCAACTATTTACACTGGTA

CTCTCTGGGAGAAAGCTCGGAA

CK149086*

60-55

0.45

1.5

0.15

0.3

AGLC15

ACTGATCAAGGTCTCTTCTAGACA

CCCAACAAACTGGACAAAGCAGA

CK149086*

60-55

0.45

1.5

0.15

0.3

AGLC16

GAGTACTTGCCAACTAGCTTAGGA

TTGGATATAACAGATGACGGGGAA

CK149087*

60-55

0.3

1.5

0.15

0.3

AGLC19

GCATCCTTCCCACTTCTTTGCA

GAATGGACTCGGATGTCTTAAGCA

CK148924*

60-55

0.3

1.5

0.15

0.3

AGLC20

AATGGTGATTCGTCAGTCGCCTA

CTGTCTGAAGAAAGTGAACGAA

CK148978*

60-55

0.3

1.5

0.15

0.3

AGLC27

CAAATTTCTGTTCTTCCACCCCAA

GGCGATCTTCGAGTCCATCGA

CK148933

60-55

0.3

1.5

0.15

0.3

AGLC28

GCTAAACCTTAGAGCAATGACTCA

CCTTGCTTGTGCCTTATCTTCCA

CK148934

60-55

0.3

1.5

0.15

0.3

AGLC29

TCTTCAACACCTCCATCTAACCTA

GACATGAAACCAAAGCATCACA

CK148945*

60-55

0.3

1.5

0.15

0.3

AGLC34

CTTTACCAAAACCACCTTCACCAA

TCTCTCTCTCTCTCTTCTGTTCCA

CK149140*

60-55

0.5

1.5

0.15

0.3

AGLC39

GCCGAGGTACACTTTACCAA

TCCTCACACTTCAGGTTCAACGA

CK149136

55-45

0.5

1.5

0.15

0.3

AGLC45

CTCCTCTTCTCCGTCGTAGCA

CTGGTCCTTGACGGGAGTGA

CK149070*

65-60

0.5

1.5

0.15

0.3

AGLC47

GTTTACATCATGACCCGCCCTA

TCACCAAGACCAGAACGTTCCA

CK148819

60-55

0.5

1.5

0.15

0.3

AGLC48

TGCCCAACGGTTTCTTTTACCA

TCAGAGATACTCGCCCACCAA

CK148960

55-45

0.5

1.5

0.15

0.3

AGLC51

TCTTTGAGCAGCATTCATTCCACA

GAGTGCTACCTTCAAAGACTGCA

CK148677

55-45

0.5

1.5

0.15

0.3

AGLC52

CGATCAAGAACCCAGTTTTGCAA

AAGATCGACAGGCGATCTGGTA

CK148718

55-45

0.5

1.5

0.15

0.3

AGLC53

CACTCTCCGTTCCGGTTCCA

CTGTCCATGCCCTTGTCCA

CK148806

60-55

0.3

1.5

0.2

0.5

AGLC55

CAGGTCGCGTTGTTGCA

GGCCGAGGTACACTTTTCCA

CK149133*

60-55

0.5

2

0.1

0.3

AGLC57

TTCATCTGGCACTAGCATATCTGA

CGACAATTCTTGCTTCAACAACCA

CK148900*

60-55

0.3

2

0.1

0.3

AGLC60

CATGTTTTCTACCCTCACAATGCA

TACTCACTTGTTGTTCCAGACA

CK149124*

55-45

0.2

1

0.1

0.2

AGLC61

TTCGATCCTCCGACCCCGAA

TTCGCTAGATCTGGATACTTCTCA

CK148802

60-55

0.3

2

0.1

0.3

AGLC64

TCTTCTTCTTCTTCTTCAGCCACA

GTGGATTGGGAAATGTGAATGTCA

CK149128*

55-45

0.2

1

0.1

0.2

AGLC66

CCACAAAGGACGACAACAACGA

CCCAACACGAACCACACGA

CK149070

60-55

0.2

2

0.2

0.5

AGLC67

ATCCATCACAACCCTCAACTCA

CTCCGTCAACCTTTCCGCAA

CK149102*

55-45

0.3

2

0.1

0.3

AGLC68

TGTTGTCTCGCCAATTCAAAGCA

CGTTTGGTGGCATTCCTGCA

CK148906*

55-45

0.3

2

0.1

0.3

AGLC72

TTTAATTACGCGGTTTCCACGA

GAAGACTTGAGACATGGGCACA

CK148928*

55-45

0.2

1

0.1

0.2

AGLC74

CGTGGGATTGAAAAAGTTGCTA

CACTACCAGCCAAAGCACTCA

CK149006*

55-45

0.2

1

0.1

0.2

AGLC75

CAACAACAACCTATCCGAACCTCA

ACTATCCCTAACCTTCCATCACCA

CK148853

60-55

0.5

1

0.2

0.5

AGLC76

CATGAGTGGTAGTGGGAGTGGA

GTTCGTTTGAGTCGTTTACTGGAA

CK148862

60-55

0.2

2

0.2

0.5

AGLC78

TCAACAACGCTACCCGATCCAA

TTCTCAAGAGCACCACAAAAGAGA

CK149132

60-55

0.3

2

0.1

0.3

AGLC79

CGGCGGCTATATTGGTTTTGCA

TCCTAAACCCCACTTATCTCCCTA

CK149080*

60-55

0.3

2

0.1

0.3

AGLC82

TTTGTGATGGTCCTGCTCTCTCA

ACCGCTTCAGGATCAACTCGA

CK148871

60-55

0.3

2

0.1

0.3

AGLC83

TCTTCCGATCCTAAGAAAGAGCAA

ACCAATATGGAGAGCACCAGTCA

CK148894

60-55

0.3

2

0.1

0.3

AGLC84

CCACCTTCCATCTCCAATTCCAA

GACTGAATCGGAGAAGGTTTCTCA

CK149089

60-55

0.15

2

0.1

0.2

AGLC85

CCAGCTTCTAATGTAGGTCTGCA

CAGCAGCAGCAGAGAGAGCA

CK148761

60-55

0.15

1

0.1

0.2

AGLC88

ACTTGGGCGTTCAAAAATCTCA

CCATTACGATCAAAGAGCTCAGGA

CK148768

55-45

0.3

2

0.1

0.3

AGLC93

GTCCGAGCTGTGGATAGGGAA

GTTCCGCCTTCAATCCATGGAA

CK148904*

60-55

0.2

2

0.2

0.5

AGLC94

CCAACTTCCCTCATTCTTATTCCA

ACCAATTCCAAATTTCCAGCTCGA

CK149093

55-45

0.2

1

0.1

0.2

AGLC96

TCCATATGGCTGAAGAACCCCAA

TTCTGAGGTTCAGGTAGTTCGGAA

CK149016*

60-55

0.2

2

0.2

0.5

AGLC98

CTCTTTCTTTCCCTCTAGTTTCCA

CGGCGAACTCGTGTTTGCTA

CK149016

55-45

0.2

1

0.1

0.2

AGLC101

TGTCCAAAATTGGGATCAGAGA

AGAACGACTTCAGCAGCAGCA

CK148993*

55-45

0.3

2

0.1

0.3

  1. *Genbank accession numbers of the longest EST sequence of the contig, used in primer design.
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BMC Plant Biology

ISSN: 1471-2229

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