Normal cells versus osmotic stressed cells. (a, b) Cells under normal growth conditions. Bar = 25 μm. (c, d) Cells grown in MS supplemented with 0.6 M mannitol (i.e. acclimated cells). Arrows correspond to dead cells (i.e. non-acclimated cells). Bar = 25 μm. (e) 3-D reconstruction of the vacuole after isosurface extraction from acclimated cells cultured in MS supplemented with 0.5 M sorbitol.