Figure 4From: New insights into the tonoplast architecture of plant vacuoles and vacuolar dynamics during osmotic stressCell perfusion with MS supplemented with 0.6 M mannitol. (a) A confocal fluorescent image merged with a Nomarski interference contrast image of convex plasmolysed cells. Arrowhead: transvacuolar strand. Bar = 10 μm. (b) A confocal fluorescent image merged with a Nomarski interference contrast image of a concave plasmolysed cell. n, nucleus. Bar = 10 μm. (c) A Nomarski interference contrast image of a plasmolysed cell. Hechtian strands (arrow) attach the protoplast tightly to the cell wall. n, nucleus. Bar = 5 μm.Back to article page