Vacuole continuity through gaps inside tonoplast sheets. (a, c, e) Single confocal images. The focal plane chosen corresponds to the one through the vacuoles' center. Arrowheads (>) in (c) delimit joined cells. n, nuclear region. Bars = 50 μm. (b, d, f) 3-D representation of the vacuole halves after isosurface extraction. Notice the openings in the vacuolar layers (b and d) and the many transvacuolar strands emanating from the nuclear region (f).