Figure 3From: New insights into the tonoplast architecture of plant vacuoles and vacuolar dynamics during osmotic stressVacuole continuity through gaps inside tonoplast sheets. (a, c, e) Single confocal images. The focal plane chosen corresponds to the one through the vacuoles' center. Arrowheads (>) in (c) delimit joined cells. n, nuclear region. Bars = 50 μm. (b, d, f) 3-D representation of the vacuole halves after isosurface extraction. Notice the openings in the vacuolar layers (b and d) and the many transvacuolar strands emanating from the nuclear region (f).Back to article page