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Table 6 Statistics for optimal primer candidates

From: Expressed sequence tags (ESTs) and simple sequence repeat (SSR) markers from octoploid strawberry (Fragaria × ananassa)

  Inside ORF Outside ORF
2 bp 35 (24.6%) 21 (31.8%)
3 bp 77 (54.2%) 14 (21.2%)
4 bp 29 (20.4%) 26 (39.4%)
5 bp 1 (0.7%) 5 (7.6%)
Total 142 (68.3%) 66 (31.7%)