Skip to main content

Table 1 List of identified xylem sap proteins from Brassica napus. Identifications of xylem sap proteins from the 2-DE gel shown in Figure 2. Sequences were determined from fragmentation spectra obtained by ESI-Q-TOF tandem MS. The resulting partial amino acid sequences were used for database searches with a BLAST algorithm optimized for short sequences. The isobaric amino acids isoleucine (I) and leucine (L), although not distinguishable by MS, are displayed as specified in the corresponding database sequences. BLAST E-values were determined using all non-redundant peptide sequences from one protein spot for another BLAST search. Bold letters in the partial sequences indicate amino acids identical to the database entry. Results of N-terminal secretion sequence predictions are indicated in the right column (Y= secretion sequence predicted, N= no secretion sequence predicted), * indicates that the observed molecular mass was lower and ** indicates that the observed mass was higher than expected.

From: Analysis of xylem sap proteins from Brassica napus

Spot No

Similarity to

Organism

Acc. No.

Sequence

identity

E value

expected Mr

observed Mr

aS

1

Ubiquitin

Arabidopsis thaliana

At2g47110

TITLEVESSDTIDNVK

16/16 (100%)

9.00E-08

8525

12064

N

2

Ubiquitin

Arabidopsis thaliana

At2g47110

TITLEVESSDTIDNVK

16/16 (100%)

9.00E-08

8525

11944

N

3

Glycine-rich cell wall protein

Arabidopsis thaliana

At4g30450

AGSSAGSFAGSR

12/12 (100%)

0.011

9530

13863

Y

4

Pathogenesis-related protein PR1a

Brassica napus

AAB09587

SPQDYVNAHNQAR

13/13 (100%)

7.00E-39

17532

18145

Y

    

LAAYAQNYADR

11/11 (100%)

    
    

QDSPQDYVNAHNQAR

15/15 (100%)

    
    

GPVQWDGTLAAYAQNYADR

19/19 (100%)

    
    

AGSSADFSGVSAVNLWVNEK

20/20 (100%)

    

5

Glycine-rich cell wall protein

Oryza sativa

BAD62284

GGGYGEGGGYGGG

15/16 (94%)

2.00E-10

14152

17822

Y

    

GGGYWGGGYGGGYGGG

     

6

Invertase/pectin methylesterase inhibitor

Arabidopsis thaliana

At1 g47960

GSSADTSGLALILVDK

15/16 (94%)

2.00E-32

22326

18297

Y

    

GVIDAGVEAAV

10/11 (91%)

    
    

ETPDFNLCVACPLDSDPR

15/18 (83%)

    
    

GSSADTSGLALILVDKIK

17/18 (94%)

    
    

ADVPEAIECCSK

10/12 (83%)

    
    

FGEDGVIDAGVEAAV

14/15 (93%)

    

7

Papain-type cysteine proteinase XCP2

Arabidopsis thaliana

At4g35350

LDHGVAAVGYGSSK

14/14 (100%)

4.00E-18

39618

16,603*

Y

    

ALAHQPVSVAIEASGR

16/16 (100%)

    
    

GVDLDHGVAAVGYGSSK

16/17 (94%)

    

8

Papain-type cysteine proteinase XCP2

Arabidopsis thaliana

At4g35350

LDHGVAAVGYGSSK

14/14 (100%)

4.00E-18

39618

16,977*

Y

    

ALAHQPVSVAIEASGR

16/16 (100%)

    
    

GVDLDHGVAAVGYGSSK

16/17 (94%)

    

9

Invertase/pectin methylesterase inhibitor

Arabidopsis thaliana

At1g47960

GSSADTSGLALILVDK

15/16 (94%)

9.00E-38

22326

18297

Y

    

ETPDFNLCVACPLDSDPR

15/18 (83%)

    
    

GSSADTSGLALILVDKIK

17/18 (94%)

    
    

ALDECASR

6/8 (75%)

    
    

ADVPEAIECCSK

10/12 (83%)

    
    

FGEDGVIDAGVEAAV

14/15 (93%)

    

10

Glycine-rich protein

Arabidopsis thaliana

At4g30460

GEGGGYGGGYGGGGD

14/15 (93%)

1.00E-04

13738

19195

Y

11

Aspartyl protease family protein

Arabidopsis thaliana

At5g07030

LPPSAIAFNPATGAGTIFDSGTVYTR

24/26 (92%)

3.00E-15

46919

21,384*

Y

    

VLIDLPNSR

7/9 (77%)

    

12

Disease resistance response/ dirigent protein

Arabidopsis thaliana

At1g64160

VIFDDPVTLDK

8/12 (67%)

3.00E-13

20573

22711

Y

    

GCLNIMGADL

9/10 (90%)

    
    

DLSVVGGTGDFFFSR

14/15 (93%)

    

13

Disease resistance response/ dirigent protein

Arabidopsis thaliana

At4g23690

VIFDDPVTLDQNYLSKPVSR

17/20 (85%)

1.00E-11

21412

24544

Y

    

SVVGGTGDFFMSR

13/13 (100%)

    

14

Endochitinase class I, putative

Arabidopsis thaliana

At2g43620

RDTIANVVTLSVFNSIFSK

12/19 (63%)

2.00E-08

30378

25856

Y

    

QAFISAAAQSSDAYK

13/15 (87%)

    
    

FNGLPLLTDPDMVSR

13/15 (87%)

    

15

Endochitinase class I, putative

Arabidopsis thaliana

At2g43620

RDTIANVVTLSVFNSIFSK

12/19 (63%)

2.00E-08

30378

25969

Y

    

QAFISAAAQSSDAYK

13/15 (87%)

    
    

FNGLPLLTDPDMVSR

13/15 (87%)

    

16

Endochitinase class I, putative

Arabidopsis thaliana

At2g43620

RDTIANVVTLSVFNSIFSK

12/19 (63%)

7.00E-13

30378

25744

Y

    

QAFISAAAQSSDAYK

13/15 (87%)

    
    

FNGLPLLTDPDMVSR

13/15 (87%)

    
    

PVLDQGFGATTR

12/12 (100%)

    

17

Germin-like protein, subfamily 2

Arabidopsis thaliana

At1 g02335

YDPDALQDLCVAADK

14/15 (93%)

8.00E-07

23441

26543

Y

    

KIPGLNTLSV

10/10 (100%)

    
    

GEVFVFPR

8/8 (100%)

    

18

Thaumatin-like protein/ osmotin

Brassica rapa

AAN23104

RAPNTLAEYALK

12/12 (100%)

4.00E-04

incomplete

32060

 

19

Gamma-glutamyltranspeptidase 1

Arabidopsis thaliana

At4g39640

PPPAPANFIRPGK

13/13 (100%)

4.00E-D5

61190

26,312*

Y

    

VPPPAPANFIRPGK

14/14 (100%)

    
    

QFIVQES

7/7 (100%)

    

20

Endochitinase class I, putative

Arabidopsis thaliana

At2g43620

TIANVVTLSVFNSIFSK

10/17 (59%)

2.00E-20

30378

28613

Y

    

QAFISAAAQSSDAYK

13/15 (87%)

    
    

FLGLPLLTDPDFVAR

12/15 (80%)

    
    

IQITSDNYNYGAAGK

12/17 (71%)

    

21

Xyloglucan:xyloglucosyl transferase

Arabidopsis thaliana

At5g57560

GQITNDGELLTLSLDK

14/16 (88%)

3.00E-36

32093

29928

Y

    

LVPGNSAGTVTTLYLK

16/16 (100%)

    
    

WFDPTWFHTYTILSDNPQR

16/19 (84%)

    
    

IFTVDGTPIR

10/10 (100%)

    
    

NFESVGTLFPNNKPFR

13/16 (81%)

    

22

Xyloglucan:xyloglucosyl transferase

Arabidopsis thaliana

At5g57560

GQITNDGELLTLSLDK

14/16 (88%)

3.00E-36

32093

30200

Y

    

LVPGNSAGTVTTLYLK

16/16 (100%)

    
    

WFDPTVVFHTYTILSDNPQR

16/19 (84%)

    
    

IFTVDGTPIR

10/10 (100%)

    
    

NFESVGTLFPNNKPFR

13/16 (81%)

    

23

Chitinase class IV, putative

Arabidopsis thaliana

At2g43570

PSFGSSISK

8/9 (89%)

2.00E-05

29775

31326

Y

    

AAANSYPSFGSSISK

14/15 (93%)

    
    

LNENLLASPEK

10/11 (91%)

    
    

VAQDPPSAFK

7/10 (70%)

    

24

Peroxidase 25 precursor

Arabidopsis thaliana

At2g41480

STVESHFDSDPTISPGLLR

19/19 (100%)

4.00E-09

35886

32665

Y

    

EASNLPSPLDSVAVQK

16/16 (100%)

    
    

VALDIGSPSNFDVSFFK

15/17 (88%)

    

25

Legume lectin family protein

Arabidopsis thaliana

At3g16530

LAVEFDTFQNK

10/11 (91%)

4.00E-06

30509

32360

Y

    

GYWVQTR

7/7 (100%)

    

Spot No

Similarity to

Organism

Acc. No.

Sequence

identity

E value

expected Mr

observed Mr

aS

26

Legume lectin family protein

Arabidopsis thaliana

At3g15356

GDNLFFLGDAELG

11/13(85%)

7.00E-15

29749

32819

Y

    

EFDTFKNK

7/8 (88%)

    
    

HVGININSMTSNVAEK

15/16 (94%)

    
    

TITIAPENVK

10/10 (100%)

    

27

Chitinase class I

Brassica napus

CAA43708

DSFINAANTFPNFANSVTR

19/19 (100%)

5.00E-11

28733

30200

Y

    

NGGNSGAVNAR

11/11 (100%)

    

28

Legume lectin family protein

Arabidopsis thaliana

At3g16530

LAVEFDTFQNK

9/10 (90%)

5.00E-06

30509

31763

Y

    

AGYWVQTR

8/8 (100%)

    

29

Chitinase class I, putative

Arabidopsis thaliana

At4g01700

GFYPYEAFVEATR

13/13 (100%)

1.00E-08

31464

32819

Y

    

GPIQLSWNYNYGQAGR

16/16 (100%)

    

30

Peroxidase, putative

Arabidopsis thaliana

At4g36430

PGTVSCAADLLTLAAR

14/16 (88%)

4.00E-05

36164

34420

Y

    

QNLSVLDIVSAAK

10/13 (77%)

    
    

GNISPLTGSSGEIR

14/14 (100%)

    

31

Peroxidase, putative

Arabidopsis thaliana

At4g36430

DSSVLTGGPCWVVP

13/14 (93%)

7.00E-18

36164

36508

Y

    

QGLDITDLVALSGSHTIGFSR

21/21 (100%)

    
    

NSDQVLFSS

9/9 (100%)

    
    

GNISPLTGSSGEIR

14/14 (100%)

    

32

Peroxidase, putative

Arabidopsis thaliana

At1g49570

EAVVLTGGPFMAPVPLGR

16/18 (89%)

9.00E-06

38030

41164

Y

    

GQPDPNLAASSDLLSK

15/16 (94%)

    
    

GNIGVFTGSDGVIR

13/14 (93%)

    

33

Peroxidase ATP5a

Arabidopsis thaliana

At1g49570

EAVVLTGGPFMAPVPLGR

16/18 (89%)

7.00E-05

38030

40065

Y

    

GNIGVFTGSDGVIR

13/14 (93%)

    
 

Peroxidase 59 precursor

Arabidopsis thaliana

At5g19890

LVEAYSQSQSLFFR

13/14 (93%)

6.00E-05

35023

40065

Y

34

Serine carboxypeptidase S10 family protein

Arabidopsis thaliana

At3g17180

GFIVGNPLTDDEYDNK

16/16 (100%)

1.00E-08

54145

40,498*

Y

35

Curculin-like (mannose-binding) lectin

Arabidopsis thaliana

At1g78830

PLVYGGDSPNHDFR

11/14 (79%)

2.00E-09

50343

39,640

Y

    

VVNNDEFGDYITEYDASYR

16/19 (84%)

    
    

GVTGFEILPNGNIVLHDK

17/18 (94%)

    
    

TGQPLVYGGDSPNHDFR

13/17 (76%)

    

36

Curculin-like (mannose-binding) lectin

Arabidopsis thaliana

At1g78830

PLVYGGDSPNHDFR

11/14 (79%)

2.00E-09

50343

36,326*

Y

    

VVNNDEFGDYITEYDASYR

16/19 (84%)

    
    

GVTGFEILPNGNIVLHDK

17/18 (94%)

    
    

TGQPLVYGGDSPNHDFR

13/17 (76%)

    

37

Peroxidase, putative

Arabidopsis thaliana

At4g33420

PTLSAGLIR

8/9 (89%)

1.00E-17

35975

37062

Y

    

NDFDNAYFNALQR

13/13 (100%)

    
    

SGVLFSDQTLFNSPFTR

15/17 (88%)

    

38

Curculin-like (mannose-binding) lectin

Arabidopsis thaliana

At1g78830

PLVYGGDSPNHDFR

11/14 (79%)

3.00E-08

50343

37,631*

Y

    

VVNNDEFGDYITEYDASYR

16/19 (84%)

    
    

ILPNGNIVLHDK

12/1 (100%)

    
    

TGQPLVYGGDSPNHDFR

13/17 (76%)

    

39

Curculin-like (mannose-binding) lectin

Arabidopsis thaliana

At1g78830

PLVYGGDSPNHDFR

11/14 (79%)

3.00E-08

50343

35,967*

Y

    

VVNNDEFGDYITEYDASYR

16/19 (84%)

    
    

ILPNGNIVLHDK

12/1 (100%)

    
    

TGQPLVYGGDSPNHDFR

13/17 (76%)

    

40

Curculin-like (mannose-binding) lectin

Arabidopsis thaliana

At1g78830

PLVYGGDSPNHDFR

11/14 (79%)

3.00E-08

50343

37,824*

Y

    

VVNNDEFGDYITEYDASYR

16/19 (84%)

    
    

ILPNGNIVLHDK

12/1 (100%)

    
    

TGQPLVYGGDSPNHDFR

13/17 (76%)

    

41

Curculin-like (mannose-binding) lectin

Arabidopsis thaliana

At1g78830

PLVYGGDSPNHDFR

11/14 (79%)

3.00E-08

50343

36,326*

Y

    

VVNNDEFGDYITEYDASYR

16/19 (84%)

    
    

ILPNGNIVLHDK

12/1 (100%)

    
    

TGQPLVYGGDSPNHDFR

13/17 (76%)

    

42

Peroxidase 59 precursor

Arabidopsis thaliana

At5g19890

KNAIPNINSAR

10/11 (91%)

0.003

35023

40280

Y

    

EAYSQSQSLFFR

12/12 (100%)

    

43

Peroxidase, putative

Arabidopsis thaliana

At4g33420

PTLAAGLIR

9/9 (100%)

1.00E-09

35975

37824

Y

    

NTAEKDSPANLSLR

14/14 (100%)

    
    

NDFDNAYFNALQR

13/13 (100%)

    

44

Cyclase family protein

Arabidopsis thaliana

At4g34180

DYLSFAAFDESPATHK

15/16 (94%)

1.00E-28

28384

34586

Y

    

GLDYLSFAAFDESPATHK

17/18 (94%)

    
    

EFDSSFSGFFTDGAK

13/15 (87%)

    
    

LIGLDYLSFAAFDESPATHK

19/20 (95%)

    
    

GRDIIPVEALK

11/11 (100%)

    

45

Peroxidase 21 (ATP2a)

Arabidopsis thaliana

At2g37130

DASLLLETAR

10/10 (100%)

5.00E-18

36741

35614

Y

    

PTLDPDYALYLK

11/12 (92%)

    
    

ADNGYFHEQFSR

12/12 (100%)

    
    

LLSETNPLTGDQGEIR

16/16 (100%)

    

46

Peroxidase, putative

Arabidopsis thaliana

At4g33420

PTLAAGLIR

9/9 (100%)

8.00E-40

35975

38018

Y

    

GFTPQDVVALSGAHTLGVAR

20/20 (100%)

    
    

LTTPDSSFDSSFVNTLTK

13/18 (72%)

    
    

FDNAYFNALQR

10/11 (91%)

    
    

SGVLFSDQTLFNTPATR

17/17 (100%)

    

47

Peroxidase, putative

Arabidopsis thaliana

At4g33420

DNTAEKDSPANLSLR

15/15 (100%)

1.00E-25

35975

37062

Y

    

GFTPQDVVALSGAHTLGVAR

20/20 (100%)

    
    

NDFDNAYFNALQR

12/13 (92%)

    
    

LFSDQTLFNTP

11/11 (100%)

    

48

Peroxidase 21 (ATP2a)

Arabidopsis thaliana

At2g37130

DASLLLETAR

10/10 (100%)

8.00E-09

36741

36691

Y

    

LLSETNPLTGDQGEIR

16/16 (100%)

    

49

Beta-1,3-glucanase (BG3)

Arabidopsis thaliana

At3g57240

LLLDVPNPDLQR

11/12 (92%)

3.00E-1B

30712

36691

N

    

YISVGNEVQPS

11/11 (100%)

    
    

FVLPAMQNIDR

10/11 (91%)

    
    

TYVNNLIQTVK

11/11 (100%)

    

Spot No

Similarity to

Organism

Acc. No.

Sequence

identity

E value

expected Mr

observed Mr

aS

50

Beta-1,3-glucanase (BG3)

Arabidopsis thaliana

At3g57240

LLLDVPNPDLQR

11/12(92%)

3.00E-18

30712

37250

N

    

YISVGNEVQPS

11/11 (100%)

    
    

FVLPAMQNIDR

10/11 (91%)

    
    

TYVNNLIQTVK

11/11 (100%)

    

51

Polygalacturonase inhibitor protein 2

Brassica napus

AAM94869

NFLQFDLSR

9/9 (100%)

5.00E-05

37117

40280

Y

    

LQEFDTYSYFHNK

13/13 (100%)

    

52

Peroxidase 59 precursor

Arabidopsis thaliana

At5g19890

PYLVQIVR

7/8 (88%)

2.00E-06

35023

41850

Y

    

GFEVVDTIK

8/9 (89%)

    
    

LVEAYSQSQSLFFR

13/14 (93%)

    

53

Peroxidase 59 precursor

Arabidopsis thaliana

At5g19890

PYLVQIVR

7/8 (88%)

5.00E-04

35023

43793

Y

    

LVEAYSQSQSLFFR

13/14 (93%)

    

54

Peroxidase 59 precursor

Arabidopsis thaliana

At5g19890

PYLVQIVR

7/8 (88%)

2.00E-06

35023

43793

Y

    

GFEVVDTIK

8/9 (89%)

    
    

LVEAYSQSQSLFFR

13/14 (93%)

    

55

Subtilase family protein

Arabidopsis thaliana

At1g20160

AVASAYGTFPTTVIDSK

16/17 (94%)

1.00E-17

81480

56,025*

Y

    

SILKPDITAPGVAILAAWTG

19/20 (95%)

    
    

LTYQVTVSATA

10/11 (91%)

    

56

Subtilase family protein

Arabidopsis thaliana

At1g20160

SPVYPLIHGK

10/10 (100%)

2.00E-05

81480

56,025*

Y

    

ASAYGTFPTTVIDSK

14/15 (93%)

    
    

VSVETPAYQIQVTPEK

13/16 (63%)

    
    

VFGALT

6/6 (100%)

    

57

Curculin-like (mannose-binding) lectin

Arabidopsis thaliana

At1g78850

KVPVDEQFLVVNK

10/13 (77%)

8.00E-16

49051

50573

Y

    

TDYSPIEYNPDVR

13/13 (100%)

    
    

ILENGNFVIYDSSVK

13/15 (87%)

    
    

SVNTDGPYSLVFEDK

12/15 (80%)

    
    

TPKPIAVREGYEFFSK

12/16 (75%)

    

58

Polygalacturonase/ glycoside hydrolase 28

Arabidopsis thaliana

At3g61490

DATLLAAQDLEEYPVLK

14/17 (82%)

1.00E-10

51939

56471

Y

    

DEYGIAFGFPTK

10/12 (83%)

    
    

IALGSEFSGGIEDVR

14/15 (93%)

    

59

Subtilase family protein

Arabidopsis thaliana

At1g20160

SPVYPLIHGK

10/10 (100%)

1.00E-10

81480

58,848*

Y

    

ASAYGTFPTTVIDSK

14/15 (93%)

    
    

SLHPTWCPSAIR

10/12 (83%)

    
    

VSVETPAYQIQVTPEK

13/16(81%)

    
    

LTYQVTVSAADDDV

10/14(71%)

    
    

VFGALT

6/6 (100%)

    

60

Subtilase family protein

Arabidopsis thaliana

At1g20160

SPVYPLIHGK

10/10 (100%)

1.00E-10

81480

56,025*

Y

    

ASAYGTFPTTVIDSK

14/15 (93%)

    
    

SLHPTWCPSAIR

10/12 (83%)

    
    

VSVETPAYQIQVTPEK

13/16(81%)

    
    

LTYQVTVSAADDDV

10/14(71%)

    
    

VFGALT

6/6 (100%)

    

61

Curculin-like (mannose-binding) lectin

Arabidopsis thaliana

At1g78850

KVPVDEQFLVVNK

10/13 (77%)

6.00E-11

49051

50573

Y

    

TDYSPIEYNPDVR

13/13 (100%)

    
    

ILENGNFVIYDSSVK

13/15 (87%)

    
    

SVNTDGPYSLVFEDK

12/15 (80%)

    
    

TPKPIAVREGYEFFSK

12/16 (75%)

    

62

Curculin-like (mannose-binding) lectin

Arabidopsis thaliana

At1g78850

KVPVDEQFLVVNK

10/13 (77%)

6.00E-11

49051

51999

Y

    

TDYSPIEYNPDVR

13/13 (100%)

    
    

ILENGNFVIYDSSVK

13/15 (87%)

    
    

SVNTDGPYSLVFEDK

12/15 (80%)

    
    

TPKPIAVREGYEFFSK

12/16 (75%)

    

63

Subtilase family protein

Arabidopsis thaliana

At1g20160

AVASAYGTFPTTVIDSK

16/17 (94%)

3.00E-07

81480

56,926*

Y

    

TVTNVGGDRAVYK

10/13 (77%)

    
    

VFGALT

6/6 (100%)

    

64

Polygalacturonase/ glycoside hydrolase 28

Arabidopsis thaliana

At3g61490

DEYGIAFGFPTK

10/12 (83%)

9.00E-08

51939

5786B

Y

    

IALGSEFSGGIEDVR

14/15 (93%)

    
    

WTLLAAQDLEEYPVLK

14/16 (88%)

    

65

Subtilase family protein

Arabidopsis thaliana

At1g20160

ESDVVLGGSK

9/10 (90%)

5.00E-08

81480

81188

Y

    

SPVYPLIHGK

10/10 (100%)

    
    

AVASAYGTFPTTVIDSK

16/17 (94%)

    
    

SLHPTWCPSAIR

10/12 (83%)

    
    

TVTNVGGDRAVYK

10/13 (77%)

    
    

LTYQVTVSA

8/9 (89%)

    

66

Subtilase family protein

Arabidopsis thaliana

At1g20160

ESDVVLGGSK

9/10 (90%)

5.00E-08

81480

79849

Y

    

SPVYPLIHGK

10/10 (100%)

    
    

AVASAYGTFPTTVIDSK

16/17 (94%)

    
    

SLHPTWCPSAIR

10/12 (83%)

    
    

TVTNVGGDRAVYK

10/13 (77%)

    
    

LTYQVTVSA

8/9 (89%)

    

67

Subtilase family protein

Arabidopsis thaliana

At1g20160

ESDVVLGGSK

9/10 (90%)

6.00E-07

81480

81188

Y

    

AVASAYGTFPTTVIDSK

16/17 (94%)

    
    

TVTNVGGDRAVYK

10/13 (77%)

    
    

LTYQVTVSA

8/9 (89%)

    
    

VFGALT

6/6 (100%)

    

68

Cucumisin-like serine protease (ARA12)

Arabidopsis thaliana

At5g67360

VEGASLLGFASGTAR

14/15 (93%)

1.00E-06

79415

78587

Y

    

DGVAIGAFAAFER

12/13 (92%)

    
    

PALAILGNGK

10/10 (100%)

    
    

LNYPSFAVNVDGSGAYK

16/17 (94%)

    
    

TVTFTVDSSK

10/10 (100%)

    

69

Germin-like protein

Arabidopsis thaliana

At3g05950

FTSGLNIAGNTIN

13/13 (100%)

7.00E-05

24736

76,260**

Y

    

AFQLDVNVVR

9/10 (90%)

    
Back to article page

BMC Plant Biology

ISSN: 1471-2229

Contact us