Construction of a bacterial artificial chromosome library from the spikemoss Selaginella moellendorffii: a new resource for plant comparative genomics

Table 2 Results of DNA hybridizations to determine gene copy number and the number of genome equivalents represented in the arrayed BAC library.

Gene probe:	Homologous to following plant genes (e value; % amino acid identity)¹:	Genome copy number:	Number of positive BAC clones from arrayed library:
SmGAI cDNA	GIBBERELLIC ACID INSENSITIVE (8e^-34; 43)	1	14
SmCYP98 cDNA	CYTOCHROME P450 98A3 (1e^-40; 66)	2	23
SmOTP	OXYANION TRANSLOCATION PROTEIN (1e^-36; 53)	2	16
SmSHR	SHORTROOT (3e^-45; 47)	1	7
SmZNF	Zn-FINGER PROTEIN (1e^-69; 90)	1	7
SmChlH	Mg CHELATASE SUBUNIT H (1e^-130; 85)	1	8
SmSNT	SYNTAXIN (9e^-55; 54)	1	12
S. moellendorffii chloroplast fragment²	Contains: Ribosomal protein L22 (2e^-33; 56) Ribosomal protein L2 (1e^-33; 71) Ribosomal protein S19 (2e^-31; 71)	ND	207
S. moellendorffii mitochondrial fragment²	Contains NADH DEHYDROGENASE subunit 5 (6e^-61; 71)	ND	0

¹Gene listed is based on results of blastx using the S. moellendorffii sequence as a query against the NR database and default parameters [24].
²sequence described is part of a genomic fragment <5 kb that was used as a probe.

ISSN: 1471-2229