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Table 2 Results of DNA hybridizations to determine gene copy number and the number of genome equivalents represented in the arrayed BAC library.

From: Construction of a bacterial artificial chromosome library from the spikemoss Selaginella moellendorffii: a new resource for plant comparative genomics

Gene probe: Homologous to following plant genes (e value; % amino acid identity)1: Genome copy number: Number of positive BAC clones from arrayed library:
SmGAI cDNA GIBBERELLIC ACID INSENSITIVE (8e-34; 43) 1 14
SmCYP98 cDNA CYTOCHROME P450 98A3 (1e-40; 66) 2 23
SmOTP OXYANION TRANSLOCATION PROTEIN (1e-36; 53) 2 16
SmSHR SHORTROOT (3e-45; 47) 1 7
SmZNF Zn-FINGER PROTEIN (1e-69; 90) 1 7
SmChlH Mg CHELATASE SUBUNIT H (1e-130; 85) 1 8
SmSNT SYNTAXIN (9e-55; 54) 1 12
S. moellendorffii chloroplast fragment2 Contains:
Ribosomal protein L22 (2e-33; 56)
Ribosomal protein L2 (1e-33; 71)
Ribosomal protein S19 (2e-31; 71)
ND 207
S. moellendorffii mitochondrial fragment2 Contains NADH DEHYDROGENASE subunit 5 (6e-61; 71) ND 0
  1. 1Gene listed is based on results of blastx using the S. moellendorffii sequence as a query against the NR database and default parameters [24].
  2. 2sequence described is part of a genomic fragment <5 kb that was used as a probe.